A cyclic AMP response element in the angiotensin-converting enzyme gene and the transcription factor CREM are required for transcription of the mRNA for the testicular isozyme.

The Journal of Biological Chemistry
S P KesslerG C Sen

Abstract

The angiotensin-converting enzyme (ACE) gene produces two mRNA species from tissue-specific promoters. The transcription start site of the mRNA for the smaller testicular isozyme (ACET) is located within an intron of the larger transcription unit that encodes the pulmonary isozyme (ACEP).We have previously demonstrated that a 298-base pair DNA fragment, 5' to the rabbit ACET mRNA transcription initiation site, can activate the testicular expression of a transgenic reporter gene. In the current study, using the same transgenic reporter system, we identified a putative cyclic AMP response element present within this DNA fragment to be absolutely essential for transcriptional activation. Moreover, we observed that ACET mRNA was not expressed in the testes of mice homozygous for a null mutation in the transcription factor CREM. However, in the same mice, ACEP mRNA was abundantly expressed in the lung. Our observations indicate that ACET mRNA expression in the testes is regulated by the putative cyclic AMP response element present 5' to the transcription start site and the corresponding transcription factor CREM.

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Citations

Mar 29, 2000·Molecular Reproduction and Development·M L SinclairL R Levin
Dec 5, 2003·Molecular and Cellular Endocrinology·Tim BeissbarthGünther Schütz
Mar 26, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Janette B GomosGanes C Sen
Aug 4, 1998·The EMBO Journal·R C Patel, G C Sen
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·Kristi L PetersGanes C Sen
Sep 28, 2004·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·J Xavier-NetoJ E Krieger
Apr 22, 2003·Molecular Reproduction and Development·Mizue HisanoMasami Nozaki
Feb 16, 2006·American Journal of Physiology. Endocrinology and Metabolism·John R PepperellMaricruz Merino
Jun 1, 2000·The Journal of Biological Chemistry·S P KesslerG C Sen
Aug 24, 1999·Methods : a Companion to Methods in Enzymology·R P Erickson

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