A cyclic-nucleotide-suppressible conductance activated by transducin in taste cells

Nature
S S Kolesnikov, R F Margolskee

Abstract

Taste can be divided into four primary sensations: salty, sour, sweet and bitter. Salty and sour are directly transduced by apical channels, whereas sweet and bitter utilize cyclic nucleotide second messengers. We have shown that rod transducin is present in mammalian taste receptor cells, where it is activated by a bitter receptor and in turn activates a phosphodiesterase. Here we introduce into frog taste cells peptides derived from transducin's phosphodiesterase-interaction region, which cause an inward whole-cell current in a subset of cells. We find that the peptides' effects are reversibly suppressed by IBMX and forskolin, indicative of a transducin-activated phosphodiesterase. Cyclic nucleotides suppress the whole-cell current, indicating that cyclic nucleotides may regulate taste-cell conductance. IBMX modifies taste-cell responses to two taste stimuli, implicating phosphodiesterase in taste transduction. Submicromolar cyclic nucleotides directly suppress the conductance of inside-out patches derived from the taste-cell plasma membrane, independently of protein phosphorylation. The channels are unusual in that they are suppressed, rather than activated by cyclic nucleotides. We propose that transducin, via phosphodieste...Continue Reading

References

Sep 23, 1991·FEBS Letters·S S KolesnikovG R Kalamkarov
Oct 11, 1991·The Journal of Membrane Biology·D W McBride, S D Roper
May 1, 1990·Pflügers Archiv : European journal of physiology·J M TangR S Eisenberg
Jan 1, 1987·The Journal of Membrane Biology·P Avenet, B Lindemann
Jan 28, 1988·Nature·K Tonosaki, M Funakoshi
Nov 1, 1988·Trends in Neurosciences·S C Kinnamon
Nov 1, 1989·The Journal of Membrane Biology·P Avenet, B Lindemann
Nov 1, 1994·Nature Structural Biology·N SpickofskyR F Margolskee
Aug 1, 1993·Current Opinion in Neurobiology·R F Margolskee
Aug 1, 1993·Current Opinion in Neurobiology·T A Gilbertson
Dec 1, 1993·Journal of Neurophysiology·T A CummingsS C Kinnamon
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·M BielF Hofmann

❮ Previous
Next ❯

Citations

Jul 31, 2003·Progress in Biophysics and Molecular Biology·Albertino BigianiFrancesca Fieni
Apr 12, 2002·Progress in Neurobiology·Albertino Bigiani
Oct 29, 1996·Biochimica Et Biophysica Acta·E J Helmreich, K P Hofmann
Sep 12, 2000·Current Opinion in Neurobiology·T A GilbertsonR F Margolskee
Aug 1, 1996·Current Opinion in Neurobiology·S C Kinnamon, R F Margolskee
Feb 4, 1999·Annals of the New York Academy of Sciences·T Tsunenari, A Kaneko
Apr 1, 1999·Annual Review of Physiology·M S Herness, T A Gilbertson
Apr 11, 2003·Photochemistry and Photobiology·Miroslawa WalerczykStanislaw Fabczak
Jun 6, 2002·Proceedings of the National Academy of Sciences of the United States of America·Matthew C Trudeau, William N Zagotta
Oct 1, 2005·Bioscience, Biotechnology, and Biochemistry·Shoko SawanoYukako Hayashi
Jan 29, 2000·Nature Neuroscience·B Lindemann
Oct 22, 2008·FEBS Letters·Tod R ClappSue C Kinnamon
Jan 29, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Shoji TabataHisao Iwamoto
Jan 18, 2005·Bioorganic & Medicinal Chemistry·Violetta LysiakJaroslaw Polanski
Oct 5, 2006·The Journal of Veterinary Medical Science·Shoji TabataHisao Iwamoto
Oct 1, 1996·Current Biology : CB·B Lindemann
Dec 7, 2000·Biophysical Journal·P B DetwilerB I Shraiman
Jul 30, 2014·Frontiers in Molecular Neuroscience·Rameshwar K Sharma, Teresa Duda
Aug 26, 1998·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·A I Spielman
Apr 16, 1998·Journal of Dental Research·A I Spielman
Nov 7, 2001·The Journal of Biological Chemistry·Robert F Margolskee
Jun 27, 1996·Nature·G T WongR F Margolskee
Jul 6, 1995·Nature·C S Zuker
Jan 27, 2006·Chemical Senses·Sami DamakRobert F Margolskee
Mar 3, 2006·American Journal of Physiology. Cell Physiology·Kristina R TrubeyNirupa Chaudhari
Jun 28, 2002·Physiological Reviews·U Benjamin Kaupp, Reinhard Seifert
Sep 5, 1997·The Journal of Biological Chemistry·T MisakaK Abe
Oct 19, 2001·The Journal of Biological Chemistry·Akihisa TerakitaYoshinori Shichida
Mar 29, 2020·Sensors·Elena von MolitorTiziana Cesetti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.