A cysteine protease that processes insect vitellin. Purification and partial characterization of the enzyme and the proenzyme.

The Journal of Biological Chemistry
X LiuJ H Nordin

Abstract

A cysteine protease that initiates degradation of vitellin (Vt) in the orthopteran Blattella germanica, and its proprotease precursor, were purified from yolk and partially characterized. The protease, purified 300-fold, contains three peptides of Mr 27,000, 29,000, and 31,000. A comparison of the purified enzyme's action pattern on Vt in vivo and in vitro confirmed its role in Vt processing. Protease-deficient yolk (day 0 postovulation) contained peptides of Mr 35,500, 37,000, 39,000, and 41,000, which were absent from yolk with protease activity. These were replaced by three peptides of approximately Mr 29,000, at days 2-3, the same time in development that protease expression and acidification of yolk granules occur (Nordin, J. H., Beaudoin, E. L., and Liu, X. (1991) Arch. Insect Biochem. Physiol. 18, 177-192). Acidification of purified proprotease converted it to three peptides of approximately Mr 29, 000 with cysteine protease activity. This conversion also required participation of a cysteine protease. Activated proprotease had the same pH activity profile, susceptibility to inhibitors, and cathepsin classification (L) as the protease. These results indicate that the Vt-processing protease is derived from a proprotease, w...Continue Reading

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Citations

Dec 17, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M MasettiF Giorgi
Sep 16, 2000·Insect Biochemistry and Molecular Biology·G RenardA Masuda
Jul 12, 2002·Insect Biochemistry and Molecular Biology·Eliane FialhoMário A C Silva-Neto
Nov 14, 2002·Insect Biochemistry and Molecular Biology·C LogulloP L Oliveira
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Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mayako KutsukakeTakema Fukatsu
Aug 17, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Andréia Bergamo EstrelaCarlos Termignoni
Sep 4, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Andréia EstrelaCarlos Termignoni
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Sep 11, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Paula C PohlAoi Masuda

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