Abstract
A cysteine protease that initiates degradation of vitellin (Vt) in the orthopteran Blattella germanica, and its proprotease precursor, were purified from yolk and partially characterized. The protease, purified 300-fold, contains three peptides of Mr 27,000, 29,000, and 31,000. A comparison of the purified enzyme's action pattern on Vt in vivo and in vitro confirmed its role in Vt processing. Protease-deficient yolk (day 0 postovulation) contained peptides of Mr 35,500, 37,000, 39,000, and 41,000, which were absent from yolk with protease activity. These were replaced by three peptides of approximately Mr 29,000, at days 2-3, the same time in development that protease expression and acidification of yolk granules occur (Nordin, J. H., Beaudoin, E. L., and Liu, X. (1991) Arch. Insect Biochem. Physiol. 18, 177-192). Acidification of purified proprotease converted it to three peptides of approximately Mr 29, 000 with cysteine protease activity. This conversion also required participation of a cysteine protease. Activated proprotease had the same pH activity profile, susceptibility to inhibitors, and cathepsin classification (L) as the protease. These results indicate that the Vt-processing protease is derived from a proprotease, w...Continue Reading
References
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J O Thomas, R D Kornberg
Apr 1, 1977·Analytical Biochemistry·J K Bryan
Jan 1, 1976·Journal of Insect Physiology·J G Kunkel, M L Pan
Jan 1, 1992·Archives of Insect Biochemistry and Physiology·R H NussenzveigH Masuda
Jan 1, 1992·Annual Review of Entomology·A S Raikhel, T S Dhadialla
Apr 15, 1992·The Biochemical Journal·B M CullenB Walker
Jun 1, 1992·The Journal of Cell Biology·S K MallyaW J Lennarz
Jan 1, 1990·Archives of Insect Biochemistry and Physiology·F Fagotto
Jan 1, 1990·Archives of Insect Biochemistry and Physiology·F Fagotto
Oct 1, 1990·Neuron·L M SchwartzC Maier
Jan 1, 1990·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M Yamashita, S Konagaya
Jan 1, 1986·Annual Review of Biochemistry·I MellmanA Helenius
Jun 1, 1988·Archives of Biochemistry and Biophysics·M MedinaC G Vallejo
Aug 1, 1986·Developmental Biology·D M WojchowskiJ G Kunkel
May 1, 1986·The Biochemical Journal·D H RichA J Barrett
Feb 1, 1986·Developmental Biology·D R ArmantW J Lennarz
Aug 15, 1970·Nature·U K Laemmli
Apr 1, 1981·Analytical Biochemistry·W N Burnette
Sep 1, 1984·Analytical Biochemistry·R B Trimble, F Maley
Jan 1, 1981·Methods in Enzymology·A J Barrett, H Kirschke
Jan 1, 1982·The Biochemical Journal·A J BarrettK Hanada
Feb 6, 1981·Biochimica Et Biophysica Acta·T D Butters, R C Hughes
Jun 1, 1994·The Journal of Cell Biology·F Fagotto, F R Maxfield
Sep 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Y Yamamoto, S Y Takahashi
Oct 1, 1995·Insect Biochemistry and Molecular Biology·P E Ribolla, A G De Bianchi
Citations
Jul 11, 1998·Archives of Insect Biochemistry and Physiology·X Liu, J H Nordin
Aug 2, 2001·The Journal of Experimental Zoology·U AbduA Sagi
Apr 28, 2004·Insect Biochemistry and Molecular Biology·Leonardo A AbreuCarlos Logullo
May 29, 2003·Journal of Insect Physiology·Y YamahamaS Y Takahashi
Dec 17, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M MasettiF Giorgi
Sep 16, 2000·Insect Biochemistry and Molecular Biology·G RenardA Masuda
Jul 12, 2002·Insect Biochemistry and Molecular Biology·Eliane FialhoMário A C Silva-Neto
Nov 14, 2002·Insect Biochemistry and Molecular Biology·C LogulloP L Oliveira
Nov 18, 1998·Insect Biochemistry and Molecular Biology·H L HandleyJ T Bradley
Dec 28, 1999·Insect Biochemistry and Molecular Biology·C Girard, L Jouanin
Apr 16, 2003·Micron : the International Research and Review Journal for Microscopy·Antonella CecchettiniFranco Giorgi
Dec 20, 2007·Arthropod Structure & Development·A CecchettiniF Giorgi
Feb 5, 2009·PLoS Neglected Tropical Diseases·Louise FordSara Lustigman
Jul 28, 2004·Proceedings of the National Academy of Sciences of the United States of America·Mayako KutsukakeTakema Fukatsu
Oct 30, 2001·Journal of Economic Entomology·K Zhu-Salzman, R A Salzman
Aug 17, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Andréia Bergamo EstrelaCarlos Termignoni
Sep 4, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Andréia EstrelaCarlos Termignoni
Jan 5, 2011·Medical and Veterinary Entomology·T Erban, J Hubert
Jun 30, 2006·Biochemical and Biophysical Research Communications·Cassia A LimaAparecida S Tanaka
Aug 24, 2001·Developmental Biology·K UchidaE Kominami
May 24, 2008·Journal of Insect Physiology·Danielle M P OliveiraEdnildo A Machado
May 5, 2011·Journal of Insect Physiology·Marcelo N MedeirosEdnildo A Machado
Feb 25, 2015·PloS One·Alexandre S MouraA Tania Bijovsky
Mar 21, 2007·Journal of Insect Physiology·Sheng-Zhang DongShusheng Liu
May 1, 1999·The Journal of Biological Chemistry·W L ChoA S Raikhel
Jun 28, 2000·Electrophoresis·S KanayaK Katsura
Jul 22, 2006·The Journal of Biological Chemistry·Sarwar HashmiSara Lustigman
Nov 8, 2002·Insect Molecular Biology·X-F ZhaoH Wieczorek
Mar 15, 2019·Parasites & Vectors·Tian-Tian ZhangJing-Ze Liu
Jan 17, 2021·Insects·Ran RosenMurad Ghanim
Oct 1, 2004·Journal of Cell Science·Collette Britton, Linda Murray
Mar 31, 2005·Archives of Biochemistry and Biophysics·Eliane FialhoMário A C Silva-Neto
Jun 1, 1997·Tissue & Cell·A M FaustoF Giorgi
Jun 1, 1997·Tissue & Cell·F GiorgiJ H Nordin
Aug 14, 2001·European Journal of Cell Biology·A CecchettiniF Giorgi
Sep 11, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Paula C PohlAoi Masuda