PMID: 13549503May 25, 1958Paper

A cytochemical study on the pancreas of the guinea pig. II. Functional variations in the enzymatic activity of microsomes

The Journal of Biophysical and Biochemical Cytology
P Siekevitz, G E Palade

Abstract

Microsomes were isolated from the pancreas of starved and fed guinea pigs. In the first case, the gland was removed from animals starved for 48 hours; in the second, the pancreas was excised 1 hour after the beginning of a meal that ended a fast of 48 hours. These are referred to below as fed animals. In both cases the tissue was homogenized in 0.88 M sucrose and the microsomes obtained by centrifuging the mitochondrial supernatant at 105,000 g for 60 minutes. In starved animals the content of the endoplasmic reticulum of the exocrine cells and the content of the microsomes were found to be of low or moderate density. In fed guinea pigs the cavities of the reticulum frequently contained dense intracisternal granules and the microsomes were distinguished by a content of high density sometimes in the form of recognizable intracisternal granules. In starved animals, the microsomes were found to account for 5 to 20 per cent of the trypsin-activatable proteolytic activity and ribonuclease activity of the whole cell, whereas in fed animals they contained uniformly almost 30 per cent of these activities. In fed animals the dense, cohesive content of the microsomes (intracisternal granules) could be isolated by breaking up the microsom...Continue Reading

References

Nov 20, 1953·The Journal of General Physiology·V ALLFREYA E MIRSKY
Mar 25, 1956·The Journal of Biophysical and Biochemical Cytology·G E PALADE, P SIEKEVITZ
Nov 25, 1956·The Journal of Biophysical and Biochemical Cytology·G E PALADE, P SIEKEVITZ
Mar 25, 1958·The Journal of Biophysical and Biochemical Cytology·P SIEKEVITZ, G E PALADE

❮ Previous
Next ❯

Citations

Sep 22, 1975·Zeitschrift Für Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology·K TóthJ Sugár
Jan 1, 1969·Zeitschrift für Zellforschung und mikroskopische Anatomie·A F Baradi, D J Brandis
Mar 25, 1965·Zeitschrift für Zellforschung und mikroskopische Anatomie·R G KESSEL
Jan 1, 1960·Zeitschrift für Zellforschung und mikroskopische Anatomie·D DUNCAN, W HILD
Jan 1, 1966·Zeitschrift für Zellforschung und mikroskopische Anatomie·R G Kessel
Aug 1, 1982·Experimental and Molecular Pathology·Y Muraoka
Aug 1, 1992·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·D Sottovia-Filho, R Taga
Jan 23, 1964·The New England Journal of Medicine·J B CAULFIELD
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R G PainterS J Singer
Nov 1, 1961·The Journal of Biophysical and Biochemical Cytology·B L MUNGER, S W BRUSILOW
Jan 1, 1962·The Journal of Cell Biology·J CHAUVEAUJ SCHNEEBELI
Apr 1, 1962·The Journal of Cell Biology·H W BEAMS, R KESSEL
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·P SIEKEVITZ, G E PALADE
Jan 25, 1959·The Journal of Biophysical and Biochemical Cytology·E Y LASFARGUESE C POLLARD
Aug 1, 1971·The Journal of Cell Biology·N B Berg, R W Young
Oct 1, 1959·The Journal of Biophysical and Biochemical Cytology·C M REDMAN, L E HOKIN
Dec 1, 1959·The Journal of Biophysical and Biochemical Cytology·H E KARRER
Jun 1, 1960·The Journal of Biophysical and Biochemical Cytology·J S ROTH
Jun 1, 1960·The Journal of Biophysical and Biochemical Cytology·D BRANDES, A PORTELA
Jun 1, 1960·The Journal of Biophysical and Biochemical Cytology·D BRANDES, A PORTELA
Jul 1, 1960·The Journal of Biophysical and Biochemical Cytology·P SIEKEVITZ, G E PALADE
Jul 1, 1983·Acta Pathologica Japonica·T Kodama, W Mori
Dec 1, 1968·Acta Physiologica Scandinavica·B Arnesjö, H Filipek-Wender
Feb 1, 1971·Acta Physiologica Scandinavica·B Arnesjö
Sep 18, 1975·Virchows Archiv. A, Pathological Anatomy and Histology·W Bieger, H F Kern
Mar 31, 2010·The Journal of Cell Biology·David D Sabatini
Apr 15, 1975·Annals of the New York Academy of Sciences·D L Drabkin
Mar 1, 1964·Journal of Neurochemistry·L M SEMINARIOC J GOMEZ
Jul 1, 1962·Journal of Neurochemistry·R K DATTA, J J GHOSH
Jun 1, 1969·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·B Flaks, J A Moody
Nov 1, 1964·The Journal of Pediatrics·W S CHERNICKG J BARBERO
Jan 14, 2017·Molecular Biology of the Cell·Karl S Matlin, Michael J Caplan
Mar 1, 1963·Biochemistry·P J KELLERR D WADE
May 1, 1978·Biological Reviews of the Cambridge Philosophical Society·R M Case
Jan 30, 1960·Nature·M FELDMANA GLOBERSON

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Biophysical and Biochemical Cytology
G E Palade
The Journal of Biophysical and Biochemical Cytology
G E Palade, P Siekevitz
The Journal of Biophysical and Biochemical Cytology
Y MOULEJ CHAUVEAU
© 2022 Meta ULC. All rights reserved