A cytochrome b5-containing plastid-located fatty acid desaturase from Chlamydomonas reinhardtii.

Eukaryotic Cell
Simone ZäunerChristoph Benning

Abstract

Monogalactosyldiacylglycerol (MGDG) in Chlamydomonas reinhardtii and other green algae contains hexadeca-4,7,10,13-tetraenoic acid (16:4) in the glycerol sn-2 position. While many genes necessary for the introduction of acyl chain double bonds have been functionally characterized, the Δ4-desaturase remained unknown. Using a phylogenetic comparison, a candidate gene encoding the MGDG-specific Δ4-desaturase from Chlamydomonas (CrΔ4FAD) was identified. CrΔ4FAD shows all characteristic features of a membrane-bound desaturase, including three histidine boxes and a transit peptide for chloroplast targeting. But it also has an N-terminal cytochrome b(5) domain, distinguishing it from other known plastid desaturases. Cytochrome b(5) is the primary electron donor for endoplasmic reticulum (ER) desaturases and is often fused to the desaturase domain in desaturases modifying the carboxyl end of the acyl group. Difference absorbance spectra of the recombinant cytochrome b(5) domain of CrΔ4FAD showed that it is functional in vitro. Green fluorescent protein fusions of CrΔ4FAD localized to the plastid envelope in Chlamydomonas. Interestingly, overproduction of CrΔ4FAD in Chlamydomonas not only increased levels of 16:4 acyl groups in cell ext...Continue Reading

References

Nov 15, 1977·The Biochemical Journal·R CammackL J Rogers
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 31, 1997·The Journal of Biological Chemistry·D HaakT Dunn
Jul 18, 2001·Journal of Experimental Botany·O SayanovaJ A Napier
May 10, 1994·Proceedings of the National Academy of Sciences of the United States of America·H WadaN Murata
Jan 23, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·P SperlingE Heinz
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jun 28, 2005·The FEBS Journal·Thierry TononIan A Graham
Dec 13, 2006·The Journal of Biological Chemistry·Markus FritzErnst Heinz
Oct 13, 2007·Science·Sabeeha S MerchantArthur R Grossman
Nov 28, 2008·The Plant Journal : for Cell and Molecular Biology·Juliane NeupertRalph Bock
Dec 5, 2008·The Plant Journal : for Cell and Molecular Biology·Attila MolnarDavid Baulcombe
Mar 31, 2009·Journal of the American Dietetic Association·Natalie D RiedigerMohammed H Moghadasian
Apr 30, 2009·Annual Review of Phytopathology·Aardra Kachroo, Pradeep Kachroo
May 7, 2009·The Journal of Biological Chemistry·Binbin Lu, Christoph Benning
May 9, 2009·BMC Genomics·Alan L KwanGary D Stormo
Aug 18, 2009·The Plant Journal : for Cell and Molecular Biology·Jinpeng GaoChristoph Benning
Oct 22, 2009·Traffic·Verena KriechbaumerBen M Abell
Nov 3, 2009·Progress in Lipid Research·Jacques JoyardNorbert Rolland

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Citations

Mar 19, 2015·Marine Drugs·Lina-Juana Dolch, Eric Maréchal
Dec 3, 2015·Proceedings of the National Academy of Sciences of the United States of America·Wenqiang YangArthur R Grossman
Mar 5, 2016·Frontiers in Plant Science·Pei-Li ShenSong Xue
Sep 25, 2015·The Plant Journal : for Cell and Molecular Biology·Rouhollah BarahimipourRalph Bock
Oct 27, 2015·The Plant Journal : for Cell and Molecular Biology·Jaruswan WarakanontChristoph Benning
Sep 18, 2012·Current Opinion in Biotechnology·Bensheng Liu, Christoph Benning
Dec 24, 2015·Frontiers in Microbiology·Javier A GimpelStephen P Mayfield
Feb 24, 2015·The Plant Journal : for Cell and Molecular Biology·Robert E Jinkerson, Martin C Jonikas
Dec 3, 2014·Biotechnology Advances·Stamatia BellouGeorge Aggelis
Aug 21, 2013·Bioresource Technology·Bensheng LiuChristoph Benning
Jul 23, 2016·Frontiers in Microbiology·Nirupama MallickAkhilesh K Singh
Oct 7, 2016·Computational and Structural Biotechnology Journal·Dongdi LiColin J Jackson
Feb 24, 2019·Plant & Cell Physiology·Jaruswan WarakanontChristoph Benning
Feb 11, 2015·The Plant Journal : for Cell and Molecular Biology·Yonghua Li-BeissonWayne Riekhof
Sep 29, 2019·Cells·Adrien BurlacotYonghua Li-Beisson
Aug 23, 2019·The Plant Journal : for Cell and Molecular Biology·Michela CecchinMatteo Ballottari
Nov 30, 2019·Functional & Integrative Genomics·Mohammad Fazel Soltani GishiniDavid Hildebrand
May 13, 2014·Molecular Biology Reports·Fatemeh Dehghan Nayeri, Kazem Yarizade
Jan 18, 2020·Plants·Valeria VecchiLuca Dall'Osto
Aug 29, 2013·The Journal of Biological Chemistry·Eugen I UrzicaSabeeha S Merchant
Feb 1, 2019·Progress in Lipid Research·Yonghua Li-BeissonJohn L Harwood

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