A cytochrome p450 conserved in insects is involved in cuticle formation.

PloS One
Tamar SztalPhillip J Daborn

Abstract

The sequencing of numerous insect genomes has revealed dynamic changes in the number and identity of cytochrome P450 genes in different insects. In the evolutionary sense, the rapid birth and death of many P450 genes is observed, with only a small number of P450 genes showing orthology between insects with sequenced genomes. It is likely that these conserved P450s function in conserved pathways. In this study, we demonstrate the P450 gene, Cyp301a1, present in all insect genomes sequenced to date, affects the formation of the adult cuticle in Drosophila melanogaster. A Cyp301a1 piggyBac insertion mutant and RNAi of Cyp301a1 both show a similar cuticle malformation phenotype, which can be reduced by 20-hydroxyecdysone, suggesting that Cyp301a1 is an important gene involved in the formation of the adult cuticle and may be involved in ecdysone regulation in this tissue.

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Citations

Apr 23, 2014·Genome Biology and Evolution·Robert T GoodCharles Robin
Mar 30, 2013·Genome Biology·Christopher I KeelingJoerg Bohlmann
Mar 29, 2014·Insect Science·Ning XuanJean-François Picimbon
Dec 1, 2015·PLoS Genetics·Michael A NajarroStuart J Macdonald
Oct 20, 2020·BMC Biology·Dorith RotenbergStephen Richards
Aug 20, 2019·Insect Biochemistry and Molecular Biology·Lixian WuSheng Li
Feb 9, 2021·Insect Biochemistry and Molecular Biology·Xiaoxi ChenQiangqiang Jia
Mar 7, 2020·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yu-Jie ZhaoNai-Yong Liu

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Methods Mentioned

BETA
PCR
restriction digest
dissections
transgenic

Software Mentioned

BLASTp
MEGA
ClustalX
Artemis

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