A cytoplasmic cell cycle controls the activity of a K+ channel in pre-implantation mouse embryos

The EMBO Journal
M L DayD I Cook

Abstract

We previously have reported that the activity of a 240 pS K+ channel varies during the cell cycle in pre-implantation mouse embryos. In the present study, we show that: (i) the cycling of channel activity is not prevented by inhibiting protein synthesis and hence does not involve cyclin-dependent kinase 1 (cdk1)-cyclin B; and (ii) the cycling of channel activity continues in anucleate zygote fragments with a time course similar to that observed in nucleate fragments. We further demonstrate that: (i) persistent activation of the K+ channel in one-cell embryos arrested in metaphase requires the maintenance of an active cdk1-cyclin B complex; and (ii) both DNA synthesis inhibition with aphidicolin and DNA damage produced by mitomycin C prevent the down-regulation of the channel at the start of S phase by a mechanism that requires tyrosine kinase activation. Thus, the 240 pS K+ channel in these cells is controlled by a previously unsuspected cytoplasmic clock that functions independently of the well-known clock controlling the chromosomal cell cycle, but can interact with it.

References

May 11, 1978·Nature·B P Fulton, D G Whittingham
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R J LeonardJ P Reuben
Jan 1, 1992·Journal of Physiology, Paris·P BregestovskiE Goyda
Jul 1, 1989·Journal of Reproduction and Fertility·C L ChatotI Torres
Oct 22, 1988·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·I R Medina, P D Bregestovski
Aug 1, 1984·The Journal of Experimental Medicine·K G ChandyS Gupta
Jan 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·K HaraM Kirschner
Jun 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·E A Nigg
Dec 1, 1994·Current Opinion in Cell Biology·S J Elledge, J W Harper
Dec 16, 1994·Science·L H Hartwell, M B Kastan
Jan 1, 1993·Progress in Biophysics and Molecular Biology·J M Dubois, B Rouzaire-Dubois
May 1, 1996·The Journal of Membrane Biology·A Lepple-WienhuesM Wiederholt
Jul 26, 1996·The Journal of Biological Chemistry·B K BerdievI I Ismailov
Dec 1, 1995·General Pharmacology·J S StroblD C Flynn
Apr 1, 1996·Current Opinion in Cell Biology·A M Pendergast
Nov 1, 1996·The Journal of Membrane Biology·W F Wonderlin, J S Strobl
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·A BrüggemannL A Pardo
Feb 1, 1997·Current Opinion in Genetics & Development·R Hansen, M Oren
Feb 1, 1997·Current Opinion in Genetics & Development·R P Fisher

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Citations

Jan 29, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M H Johnson, M L Day
Nov 13, 1998·Current Opinion in Neurobiology·O Pourquié
Mar 29, 2007·Reproduction, Fertility, and Development·Jeremy G B ThompsonKaren L Kind
Feb 28, 2003·Biology of Reproduction·David P LuChristopher O'Neill
Feb 5, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Diana UrregoLuis A Pardo
Nov 2, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·I ArnaizM L Day
Dec 10, 2002·Reproductive Biomedicine Online·M H Johnson
Jan 18, 2005·Vascular Pharmacology·Christoph Rüdiger Wolfram KuhlmannJohannes Wiecha
Jul 24, 2004·Seminars in Cell & Developmental Biology·Martin H Johnson, Josie M L McConnell
Jun 23, 2007·Experimental Cell Research·Yan LiChris O'Neill
Oct 28, 2017·The Journal of Cell Biology·Bernhard StraussJonathon Pines
Jan 25, 2005·Bulletin of Experimental Biology and Medicine·D V Gol'dshteinA A Smirnov
Jun 30, 2010·Molecular Reproduction and Development·Elisabetta Tosti
Mar 25, 2011·American Journal of Physiology. Cell Physiology·Andrea Becchetti
Feb 8, 2002·American Journal of Physiology. Cell Physiology·James R TrimarchiDavid L Keefe
Jan 13, 2006·Doklady Biological Sciences : Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections·T A Sviridova-ChailakhyanL M Chailakhyan
Mar 19, 2016·Birth Defects Research. Part C, Embryo Today : Reviews·Elisabetta TostiAlessandra Gallo
Apr 26, 2005·Doklady. Biochemistry and Biophysics·A G PogorelovN Yu Sakharova
Aug 16, 2000·Cell Calcium·M L DayM H Johnson
Mar 9, 2004·Biochemical and Biophysical Research Communications·Halima Ouadid-AhidouchNatalia Prevarskaya
Mar 11, 2003·Reproductive Biomedicine Online·R G Edwards

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