PMID: 138078Nov 24, 1976

A cytoplasmic gene for partial suppression of a nuclear pleiotropic respiratory deficient mutant in the petite negative yeast Schizosaccharomyces pombe

Molecular & General Genetics : MGG
A M ColsonA Goffeau

Abstract

The nuclear pleiotropic respiratory-deficient mutant pet1 (previously M126) exhibits cytochromes aa3 and b deficiencies accompanied by loss of the oligomycin-sensitivity of the mitochondrial ATPase. The mutant pet1, unable to grow on glycerol, growth on glucose. The latter phenotypic trait symbolized by ANAS-D, exhibits a high frequency (2 to 4 X 10(5)) Of spontaneous suppression into Antimycin A-resistant strains. Mutagenesis with MnCl2 increases by a factor of 10(2) the frequency of ANAR-D derivatives. This suppression is partial since none of the suppressed strains is able to grow on glycerol even when respiratory functions and cytochromes activities are restored as in the pet1 [SUP2] strain. In the latter strain it is concluded that the extralocus suppressor gene [SUP2] is responsible for the ANAR-D trait. Tetrad analysis in a cross homozygous for pet1 demonstrates a non-Mendelian segregation pattern for the SUP2 suppressor gene. In stable diploids, homozygous for pet1, the [SUP2] suppressor exhibits a mitotic segregation pattern. Furthermore the transmission of the [SUP2] gene is decreased by ethidium bromide treatment. Therefore, the [SUP2] suppressor gene responsible for partial suppression of the nuclear pleiotropic phe...Continue Reading

References

May 19, 1975·Biochemical and Biophysical Research Communications·E M Storm, J Marmur
Jan 1, 1971·Molecular & General Genetics : MGG·K WolfF Kaudewitz
Mar 26, 1974·Biochimica Et Biophysica Acta·W BandlowE C Slater
Jan 1, 1972·Annual Review of Biochemistry·P Borst
Sep 12, 1973·Molecular & General Genetics : MGG·Z Kotylak
Nov 22, 1973·Molecular & General Genetics : MGG·A PutramentW Prazmo
Jan 1, 1974·Molecular & General Genetics : MGG·S Ulaszewski, T M Lachowicz
Jan 1, 1974·Molecular & General Genetics : MGG·A M ColsonJ R Mattoon
Jan 1, 1971·Molecular & General Genetics : MGG·R SchwabF Kaudewitz
Nov 25, 1968·Biochemical and Biophysical Research Communications·J H ParkerJ R Mattoon
Feb 1, 1956·Journal of General Microbiology·M B MITCHELL, H K MITCHELL
Jul 15, 1964·Biochimica Et Biophysica Acta·F SHERMAN, P P Slonimski

Related Concepts

DNA-dependent ATPase
Cochliobolus
Cytochromes
Cytoplasmic Inheritance
Schizosaccharomyces pombe
Suppressor Mutation

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