A Damage Sensor Associated with the Cuticle Coordinates Three Core Environmental Stress Responses in Caenorhabditis elegans

Genetics
William DoddKeith P Choe

Abstract

Extracellular matrix barriers and inducible cytoprotective genes form successive lines of defense against chemical and microbial environmental stressors. The barrier in nematodes is a collagenous extracellular matrix called the cuticle. In Caenorhabditis elegans, disruption of some cuticle collagen genes activates osmolyte and antimicrobial response genes. Physical damage to the epidermis also activates antimicrobial responses. Here, we assayed the effect of knocking down genes required for cuticle and epidermal integrity on diverse cellular stress responses. We found that disruption of specific bands of collagen, called annular furrows, coactivates detoxification, hyperosmotic, and antimicrobial response genes, but not other stress responses. Disruption of other cuticle structures and epidermal integrity does not have the same effect. Several transcription factors act downstream of furrow loss. SKN-1/Nrf and ELT-3/GATA are required for detoxification, SKN-1/Nrf is partially required for the osmolyte response, and STA-2/Stat and ELT-3/GATA for antimicrobial gene expression. Our results are consistent with a cuticle-associated damage sensor that coordinates detoxification, hyperosmotic, and antimicrobial responses through overla...Continue Reading

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Citations

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Datasets Mentioned

BETA
GSE107704

Methods Mentioned

BETA
environmental stress
nuclear translocation
PCR
Assay
Protein Assay
RNAseq
electron microscopy
confocal microscopy
atomic force microscopy

Software Mentioned

Clone Mapper
Gene Cluster
BIOSORT
Image J
COPAS BIOSORT
Treeview
DAVID ( the Database for Annotation , Visualization , and Inte...
Kallisto
Java
Database for Annotation , Visualization , and Integrated Disco...

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