A decade of advances in transposon-insertion sequencing.

Nature Reviews. Genetics
Amy K CainTim van Opijnen

Abstract

It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS) methods, which combine genome-wide transposon mutagenesis with high-throughput sequencing to estimate the fitness contribution or essentiality of each genetic component in a bacterial genome. Four TIS variations were published in 2009: transposon sequencing (Tn-Seq), transposon-directed insertion site sequencing (TraDIS), insertion sequencing (INSeq) and high-throughput insertion tracking by deep sequencing (HITS). TIS has since become an important tool for molecular microbiologists, being one of the few genome-wide techniques that directly links phenotype to genotype and ultimately can assign gene function. In this Review, we discuss the recent applications of TIS to answer overarching biological questions. We explore emerging and multidisciplinary methods that build on TIS, with an eye towards future applications.

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Citations

Sep 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Rachel M WheatleyPhilip S Poole
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Methods Mentioned

BETA
PCR
fluorescence sorting
density gradient centrifugation
fluorescence-activated cell sorting
FACS
RNA-seq
PCA

Software Mentioned

Tn
ShinyOmics
TRANSIT
FAST
vault
- Xpress
dTn
AlbaTraDIS
Dalliance
TraDISort

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