A Decellularized Matrix Hydrogel Derived from Human Dental Pulp Promotes Dental Pulp Stem Cells Proliferation, Migration, and Induced Multidirectional Differentiation in vitro

Journal of Endodontics
Junda LiQiting Huang

Abstract

Dental pulp is a major composition in pulp-dentin complex, which serves as protective system against dental trauma/infection. A functional dental pulp regeneration is highly desirable after pulpitis or pulp necrosis. However, endodontic regeneration has remained challenging for decades, due to the deconstructive microenvironment and lack of functional cells within root canal system. The present study developed a decellularized matrix hydrogel derived from human dental pulp (hDDPM-G), which might serve as a growth-permissive microenvironment for dental pulp regeneration. Human dental pulps extracted from healthy wisdom teeth were decellularized and digested, then underwent sol-gel transition to form hDDPM-G. The protein compositions were identified by proteomic analysis. Human dental pulp stem cells (hDPSCs) were seeded on hDDPM-G coated surfaces and evaluated by immunofluorescence staining, Transwell migration, and CCK-8 assays. Induced hDPSCs differentiation was examinedin vitroand characterized by immunostaining, western blotting, and RT-PCR. Complete decellularization was implemented. Protein contents found in hDDPM were identified to contribute in promoting cell proliferation, migration, and regulation of stem cell differen...Continue Reading

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