A Decellularized Porcine Xenograft-Derived Bone Scaffold for Clinical Use as a Bone Graft Substitute: A Critical Evaluation of Processing and Structure

Journal of Functional Biomaterials
Daniel N BraceyPatrick W Whitlock

Abstract

Bone grafts are used in approximately one half of all musculoskeletal surgeries. Autograft bone is the historic gold standard but is limited in supply and its harvest imparts significant morbidity to the patient. Alternative sources of bone graft include allografts, synthetics and, less commonly, xenografts which are taken from animal species. Xenografts are available in unlimited supply from healthy animal donors with controlled biology, avoiding the risk of human disease transmission, and may satisfy current demand for bone graft products. In the current study, cancellous bone was harvested from porcine femurs and subjected to a novel decellularization protocol to derive a bone scaffold. The scaffold was devoid of donor cellular material on histology and DNA sampling (p < 0.01). Microarchitectural properties important for osteoconductive potential were preserved after decellularization as shown by high resolution imaging modalities. Proteomics data demonstrated similar profiles when comparing the porcine bone scaffold against commercially available human demineralized bone matrix approved for clinical use. We are unaware of any porcine-derived bone graft products currently used in orthopaedic surgery practice. Results from th...Continue Reading

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Citations

Sep 29, 2019·Biomedicines·Marissa D'SouzaAllen L Ho
Sep 10, 2020·Acta Cirúrgica Brasileira·Felipe Rocha Dos SantosLuis Gustavo Gosuen Gonçalves Dias
Aug 6, 2020·International Journal of Molecular Sciences·Unai MendibilAnder Abarrategi
Nov 18, 2018·International Journal of Molecular Sciences·Patrick Frank OttensmeyerEdda Tobiasch
Nov 30, 2020·European Journal of Pharmacology·Andrzej HudeckiMarek J Łos
Jan 12, 2021·Bio-design and Manufacturing·Pengju WangHaitao Liu
Feb 25, 2021·Journal of Tissue Engineering and Regenerative Medicine·Yuan-Wu ChenHua-Hong Chien

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Methods Mentioned

BETA
xenograft
xenografts
Assay

Software Mentioned

Scanco
Mascot
ExPASy
Bluehill

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