PMID: 9180030May 5, 1997Paper

A dedicated internal standard in fragment length analysis of hyperpolymorphic short tandem repeats

Forensic Science International : Synergy
B M Dupuy, B Olaisen

Abstract

Some of the most polymorphic short tandem repeats (STRs) have alleles differing in size by as little as one basepair. Since sizing precision with commercially available internal standards ordinarily does not allow safe discrimination at this level, typing is accomplished through comparisons with allelic ladders run on each gel. Here we introduce the use of optimally spaced sequenced alleles as a dedicated internal standard for measurement and typing of hyperpolymorphic STRs. We have constructed such a dedicated internal standard for HUMACTBP2 ([1]; Moos and Gallwitz, EMBO I., 5 (1983) 757-761) typing, including 25 sequenced, ROX labelled HUMACTBP2 alleles spanning the size range for alleles at this locus (233-333 basepairs (bp)). Comparisons of inter-gel runs of selected alleles with this dedicated standard and the commercial GS500 internal standard demonstrate that only the presently described internal standard is suited for direct typing based on fragment length measurements only. Obvious advantages of this typing procedure are that fragment measurements are precise and in accordance with sequenced lengths, and that the typing procedure via an external allelic ladder is avoided. Sequences of the alleles used in the internal s...Continue Reading

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Citations

May 11, 1999·Parasitology Today·R WangL Zheng
Jun 8, 2001·Forensic Science International : Synergy·P Hoff-OlsenB Olaisen
Dec 13, 2002·Forensic Science International : Synergy·Hsing-Mei HsiehJames Chun-I Lee
Feb 23, 2005·Molecular Ecology·G L HarperW O C Symondson
Dec 4, 2012·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Julian W Tang
May 19, 2001·Electrophoresis·L C TsaiJ C Lee

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