A Deep Insight Into the Sialotranscriptome of the Chagas Disease Vector, Panstrongylus megistus (Hemiptera: Heteroptera)

Journal of Medical Entomology
José M C RibeiroIvo M B Francischetti

Abstract

Saliva of blood-sucking arthropods contains a complex cocktail of pharmacologically active compounds that assists feeding by counteracting their hosts' hemostatic and inflammatory reactions. Panstrongylus megistus (Burmeister) is an important vector of Chagas disease in South America, but despite its importance there is only one salivary protein sequence publicly deposited in GenBank. In the present work, we used Illumina technology to disclose and publicly deposit 3,703 coding sequences obtained from the assembly of >70 million reads. These sequences should assist proteomic experiments aimed at identifying pharmacologically active proteins and immunological markers of vector exposure. A supplemental file of the transcriptome and deducted protein sequences can be obtained from http://exon.niaid.nih.gov/transcriptome/P_megistus/Pmeg-web.xlsx.

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Citations

Sep 16, 2015·Insect Biochemistry and Molecular Biology·José M C RibeiroIvo M B Francischetti
Aug 16, 2016·Journal of Insect Physiology·Maurício Roberto Viana Sant'AnnaMarcos Horácio Pereira
Sep 29, 2020·Frontiers in Cellular and Infection Microbiology·Paula Beatriz SantiagoJaime Martins Santana
Feb 3, 2020·Trends in Parasitology·Paula B SantiagoJaime M Santana
Jun 9, 2020·Acta Tropica·Daiki MizushimaHirotomo Kato
Aug 22, 2020·Current Opinion in Insect Science·Pablo Emiliano Cantón, Bryony C Bonning

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