A defective signal peptide tethers the floury-2 zein to the endoplasmic reticulum membrane

Plant Physiology
Jeffrey W GillikinR S Boston

Abstract

The maize (Zea mays L.) floury-2 (fl2) mutation is associated with a general decrease in storage protein synthesis, altered protein body morphology, and the synthesis of a novel 24-kD alpha-zein storage protein. Unlike storage proteins in normal kernels and the majority of storage proteins in fl2 kernels, the 24-kD alpha-zein contains a signal peptide that would normally be removed during protein synthesis and processing. The expected processing site of this alpha-zein reveals a putative mutation alanine-->valine (Ala-->Val) that is not found at other junctions between signal sequences and mature proteins. To investigate the impact of such a mutation on signal peptide cleavage, we have assayed the 24-kD fl2 alpha-zein in a co-translational processing system in vitro. Translation of RNA from fl2 kernels or synthetic RNA encoding the fl2 alpha-zein in the presence of microsomes yielded a 24-kD polypeptide. A normal signal peptide sequence, generated by site-directed mutagenesis, restored the capacity of the RNA to direct synthesis of a properly processed protein in a cell-free system. Both the fl2 alpha-zein and the fl2 alpha-zein (Val-->Ala) were translocated into the lumen of the endoplasmic reticulum. The processed fl2 alpha-z...Continue Reading

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Citations

Jun 9, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yongrui WuJoachim Messing
Mar 31, 2005·Trends in Genetics : TIG·Bryan C Gibbon, Brian A Larkins
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·J C.M. CascardoE P.B. Fontes
Mar 6, 1999·Current Opinion in Plant Biology·K Matsuoka, S Y Bednarek
Aug 21, 2013·Nature Communications·Yongrui WuJoachim Messing
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·C E ColemanB A Larkins
Mar 11, 1999·The Plant Cell·N Leborgne-CastelJ Denecke
Feb 23, 2012·Plant Biotechnology Journal·Tejinder KumarTom Clemente
Feb 2, 2013·Biochimica Et Biophysica Acta·Julie Hollien
Jul 8, 2014·Frontiers in Plant Science·David R Holding
Apr 18, 2013·International Journal of Molecular Sciences·Yan DengStephen H Howell
Apr 15, 2006·Plant & Cell Physiology·Hiroshi YasudaFumio Takaiwa
May 18, 2013·Molecular Biology Reports·Maryam Sarwat, Afsar Raza Naqvi
Jul 7, 2018·Plant Reproduction·Shanshan ZhangRamin Yadegari

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