PMID: 3754362May 1, 1986Paper

A defined in vitro system for packaging of bacteriophage T3 DNA

Virology
K HamadaT Minagawa

Abstract

Using purified components, we have constructed an in vitro system for packaging of mature phage T3 DNA. In addition to mature T3 DNA, the system contained T3 proheads and the products of gene 18 (gp18) and gene 19 (gp19). The reaction required Mg2+, ATP, and polyvinyl alcohol. Spermidine was stimulatory but not absolutely required for the packaging reaction. Polyvinyl alcohol could be replaced by polyethylene glycol. The packaging efficiency decreased with decreasing molecular weight of the polymer, and low molecular weight polyols such as sucrose, sorbitol, and glycerol were inactive. The packaging reaction exhibited a sigmoidal relationship with respect to the concentration of ATP with the concentration for half maximal activity about 15 microM. A nonhydrolyzable ATP analog, adenosine 5'-O-(3-thiotriphosphate), inhibited the packaging reaction.

References

Jun 15, 1978·Virology·H FujisawaT Minagawa
Aug 1, 1978·Virology·J I MiyazakiT Minagawa
Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·R S FullerA Kornberg
Sep 1, 1980·Cell·W C Earnshaw, S R Casjens
Feb 1, 1981·Virology·M YamagishiT Minagawa
Feb 1, 1981·Virology·H Matsuo-KatoT Minagawa

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Citations

Aug 20, 1987·Journal of Molecular Biology·H ShibataT Minagawa
Apr 22, 2008·Quarterly Reviews of Biophysics·Elmar NurmemmedovAlex Evilevitch
Dec 31, 2011·Proceedings of the National Academy of Sciences of the United States of America·Siyang SunVenigalla B Rao
Aug 9, 2008·Annual Review of Genetics·Venigalla B Rao, Michael Feiss
May 2, 2013·The Journal of Biological Chemistry·María I DaudénJosé L Carrascosa
May 1, 1994·Quarterly Reviews of Biophysics·J M Valpuesta, J L Carrascosa
Feb 20, 2013·Virus Research·Leonor OliveiraJuan C Alonso
Jan 20, 2009·Journal of Molecular Biology·Gabija ZiedaiteDennis H Bamford
Jan 1, 1990·Virology·H FujisawaC Hashimoto
Apr 1, 1992·Virology·C Hashimoto, H Fujisawa
Oct 1, 1988·Virology·C Hashimoto, H Fujisawa
Apr 14, 2005·Journal of Molecular Biology·Nelli J StrömstenJaana K H Bamford
Nov 16, 2010·Journal of Structural Biology·Anton S Petrov, Stephen C Harvey
Oct 24, 2000·Journal of Structural Biology·J M ValpuestaJ L Carrascosa
Feb 5, 2000·Journal of Molecular Biology·A Dröge, P Tavares
Nov 25, 1998·Journal of Molecular Biology·P J Jardine, D H Coombs

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