A degradation signal recognition in prokaryotes.

Journal of Synchrotron Radiation
Eun Young Park, Hyun Kyu Song

Abstract

The degradation of ssrA-tagged substrates in prokaryotes is conducted by a subset of ATP-dependent proteases, including ClpXP complex. More than 630 sequences of ssrA have been identified from 514 species, and are conserved in a wide range of prokaryotes. SspB protein markedly stimulates the degradation of these ssrA-tagged substrates by the ClpXP proteolytic machine. The dimeric SspB protein is composed of a compact ssrA-binding domain, which has a dimerization surface and a flexible C-terminal tail with a ClpX-binding motif at its very end. Since SspB is an adaptor protein for the ClpXP complex, designed mutagenesis, fluorescence spectroscopy, biochemistry and X-ray crystallography have been used to investigate the mechanism of delivery of ssrA-tagged proteins. In this paper the structural basis of ssrA-tag recognition by ClpX and SspB, as well as SspB-tail recognition by ZBD, is described.

References

Apr 1, 1995·Current Biology : CB·B J Mayer, M J Eck
Oct 1, 1995·Genes & Development·I LevchenkoT A Baker
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·E G FrankR Woodgate
Jan 1, 1996·Annual Review of Genetics·S Gottesman
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Oct 6, 1998·Annual Review of Biochemistry·A Hershko, A Ciechanover
May 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·G BeckerR Hengge-Aronis
Jul 20, 1999·Annual Review of Biophysics and Biomolecular Structure·M BochtlerR Huber
Sep 29, 2000·Science·I LevchenkoT A Baker
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·H K SongR Huber
Mar 30, 2001·The Journal of Biological Chemistry·B BaneckiM Zylicz
Sep 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·J M FlynnT A Baker
Apr 6, 2002·Current Opinion in Microbiology·Jeffrey H Withey, David I Friedman
May 6, 2003·Genes & Development·Saskia B NeherTania A Baker
Jun 26, 2003·Nucleic Acids Research·Torsten SchwedeManuel C Peitsch
Aug 26, 2003·The Journal of Biological Chemistry·Urszula A WojtyraWalid A Houry
Sep 30, 2003·The Journal of Biological Chemistry·Dong Young Kim, Kyeong Kyu Kim
Oct 4, 2003·The Journal of Biological Chemistry·Logan W DonaldsonWalid A Houry
Nov 5, 2003·Proceedings of the National Academy of Sciences of the United States of America·Saskia B NeherTania A Baker
Mar 24, 2004·Journal of Structural Biology·Axel MogkBernd Bukau
Sep 30, 2004·Cell·Robert T SauerTania A Baker
May 10, 2005·Nature Structural & Molecular Biology·Igor LevchenkoTania A Baker
Aug 2, 2005·Nature Cell Biology·Jonathon Pines, Catherine Lindon
Aug 3, 2005·Nature Reviews. Molecular Cell Biology·Phyllis I Hanson, Sidney W Whiteheart
Jun 10, 2006·Molecular Cell·Kathleen E McGinnessRobert T Sauer
Jul 1, 2006·The EMBO Journal·Guillaume ThibaultWalid A Houry
Nov 7, 2006·Journal of Bacteriology·Faith H LessnerKenneth C Keiler
Nov 9, 2006·Proceedings of the National Academy of Sciences of the United States of America·Guillaume ThibaultWalid A Houry
Jan 30, 2007·Journal of Molecular Biology·Eun Young ParkHyun Kyu Song
Apr 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Peter ChienTania A Baker

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Citations

Nov 15, 2011·Molecules and Cells·Byung-Gil LeeHyun Kyu Song
Mar 23, 2010·Nature Structural & Molecular Biology·Byung-Gil LeeHyun Kyu Song
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Sharief BarendsGilles P van Wezel
Oct 12, 2014·Biochemical and Biophysical Research Communications·Kwang Hoon Sung, Hyun Kyu Song

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Methods Mentioned

BETA
peptide array
NMR
isothermal titration calorimetry
biosensor

Software Mentioned

tmRNA

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