PMID: 9418041Jan 7, 1998Paper

A deletion mutation at the ep locus causes low seed coat peroxidase activity in soybean

The Plant Journal : for Cell and Molecular Biology
Mark Gijzen

Abstract

The Ep locus severely affects the amount of peroxidase enzyme in soybean seed coats. Plants containing the dominant Ep allele accumulate large amounts of peroxidase in the hourglass cells of the sub-epidermis. Homozygous recessive epep genotypes do not accumulate peroxidase in the hourglass cells and are much reduced in total seed coat peroxidase activity. To isolate the gene encoding the seed coat peroxidase and to determine whether it corresponds to the Ep locus, a cDNA library was constructed from developing seed coats and an abundant 1.3 kb peroxidase transcript was cloned. The corresponding structural gene was also isolated from a genomic library. Sequence analysis shows that the seed coat peroxidase is translated as a 352 amino acid precursor protein of 38 kDa. Processing of a putative 26 amino acid signal sequence results in a mature protein of 326 residues with a calculated mass of 35 kDa and a pl of 4.4. Using probes derived from the cDNA, genomic DNA blot hybridization and polymerase chain reaction analysis detected polymorphisms that distinguished EpEp and epep genotypes. Co-segregation of the polymorphisms in an F2 population from a cross of EpEp and epep plants shows that the Ep locus encodes the seed coat peroxida...Continue Reading

Citations

Apr 6, 2004·Biochimica Et Biophysica Acta·Karen G Welinder, Yvonne B Larsen
Feb 15, 2003·Plant Physiology·Zhongming ZhangPriti Krishna
May 12, 2000·Plant Physiology·D QutobM Gijzen
Mar 27, 2001·Protein Science : a Publication of the Protein Society·A HenriksenM Gajhede
Mar 16, 2006·BMC Plant Biology·Mark GijzenPat Moy
Nov 22, 2001·Biochemical and Biophysical Research Communications·J K Kamal, D V Behere
Jun 6, 2009·Plant Biotechnology Journal·Loreta Gudynaite-SavitchBrian L A Miki
Jul 4, 2006·Trends in Biotechnology·Barry J RyanCiarán O'Fágáin
May 28, 2010·Annals of Botany·S S MillerD A Johnson
Nov 16, 2001·Journal of Experimental Botany·M GijzenJ T Chernys
Aug 3, 2005·Journal of Experimental Botany·Sangeeta DhaubhadelMark Gijzen
Jan 24, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Weican ZhangPeiji Gao
Dec 13, 2005·Carbohydrate Research·James S S Gray, Rex Montgomery
Jul 18, 2002·Biochemistry·J K Amisha Kamal, Digambar V Behere

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