A demonstration of pattern formation without positional information in Dictyostelium

Development, Growth & Differentiation
Christopher R L ThompsonRobert R Kay

Abstract

Although positional information, conveyed by morphogen gradients, is a widely accepted way of forming patterns during development, an alternative method is conceivable, based on the intermingled differentiation of cells with different fates, followed by their sorting into discrete pattern elements. It has been proposed that Dictyostelium prestalk and prespore cells behave in this way at the mound stage of development. However, it has been difficult to conclusively demonstrate that they initially differentiate intermingled, because rapid cell movement within the mound makes it impossible to be sure where prestalk and prespore cells originate. We have taken a novel approach to address this problem by blocking cell movement at different stages in development, using the actin-depolymerizing drug, latrunculin-A. Prestalk and prespore cells differentiate with essentially normal efficiency and timing in such paralyzed structures. When movement is blocked sufficiently early, the major cell types all subsequently differentiate at scattered positions throughout the aggregate, and even in the streams leading into it. Our work strongly supports the idea that the prestalk/prespore pattern in Dictyostelium forms without positional informatio...Continue Reading

References

Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D TraynorJ G Williams
Jul 13, 1989·Nature·K A JermynJ G Williams
Sep 1, 1970·The Biochemical Journal·D J Watts, J M Ashworth
Nov 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C H SiuT Y Lam
Oct 30, 1980·Nature·C Nüsslein-Volhard, E Wieschaus
Jan 1, 1981·Differentiation; Research in Biological Diversity·M Tasaka, I Takeuchi
Aug 1, 1995·Current Biology : CB·F Siegert, C J Weijer
May 1, 1994·Developmental Biology·B K Yoder, D D Blumberg
Jan 1, 1993·Current Topics in Developmental Biology·W F Loomis
Apr 7, 1999·Developmental Biology·K A Kellerman, J G McNally
Aug 4, 1999·Nature Genetics·D J Headon, P A Overbeek
Mar 9, 2000·Seminars in Cell & Developmental Biology·R R KayC R Thompson
Mar 18, 2000·Molecular Genetics and Metabolism·A VeraksaW McGinnis
Feb 13, 2001·Molecular Cell·C R Thompson, R R Kay
Aug 3, 2001·Nature Reviews. Genetics·T Tabata
Sep 11, 2002·Developmental Biology·Andrea Streit
Mar 1, 2003·Nature Genetics·Kathryn V Anderson, Philip W Ingham
Jan 20, 2004·Development·Christopher R L ThompsonGad Shaulsky

❮ Previous
Next ❯

Citations

Apr 25, 2012·Proceedings of the National Academy of Sciences of the United States of America·Tetsuya MuramotoJonathan R Chubb
Jan 19, 2013·Molecular Biology and Evolution·Xiangjun TianDavid C Queller
May 12, 2010·Cold Spring Harbor Perspectives in Biology·Robert R Kay, Christopher R L Thompson
May 19, 2011·Development, Growth & Differentiation·Joan E Strassmann, David C Queller
Jan 18, 2006·Development·Eryong HuangChristopher R L Thompson
Jan 30, 2010·Development·Michelle StevenseJonathan R Chubb
Apr 7, 2011·PLoS Biology·Katie ParkinsonChristopher R L Thompson
Feb 15, 2007·PloS One·Clément NizakRichard H Kessin
Nov 28, 2013·ELife·Alex ChattwoodChristopher R L Thompson
Mar 28, 2008·Development·Thomas Keller, Christopher R L Thompson
Jul 20, 2010·Current Opinion in Genetics & Development·Jonathan R Chubb, Tanniemola B Liverpool
Jul 7, 2009·Journal of Molecular Biology·Ritwick SawarkarVidyanand Nanjundiah
Jun 21, 2005·Developmental Biology·Lana StrmeckiCatherine J Pears
Apr 17, 2019·Biological Reviews of the Cambridge Philosophical Society·Jenks Hehmeyer
Apr 13, 2019·Development·Agnes MiermontJonathan R Chubb
May 19, 2011·Development, Growth & Differentiation·Alex Chattwood, Christopher R L Thompson
Aug 25, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Néstor Saiz, Anna-Katerina Hadjantonakis
Oct 30, 2013·Experimental Dermatology·Denis Headon
Jul 19, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Jonathan R Chubb

❮ Previous
Next ❯

Related Concepts

Related Feeds

Actin, Myosin & Cell Movement

Contractile forces generated by the actin-myosin cytoskeleton are critical for morphogenesis, but the cellular and molecular mechanisms of contraction have been elusive for many cell shape changes and movements. Here is the latest research on the roles of actin and myosin in cell movement.