A Density-Changing Centrifugation Method for Efficient Separation of Free Drugs from Drug-Loaded Particulate Delivery Systems

The AAPS Journal
Lu HanLi Deng

Abstract

Commonly used separation techniques, such as ultracentrifugation, chromatography, and membrane separation, have inherent drawbacks that limit their usage. Herein, we introduced a new separation method, density-changing centrifugation (DCC), which is based on trisodium citrate (TC) and ultracentrifugation. Paclitaxel-loaded cationic solid lipid nanoparticles (SLNs/PTX) and doxorubicin-loaded PEGylated liposomes (Lipo/Dox) were prepared as model drug delivery particulates. After optimizing TC concentration and centrifugal conditions, DCC showed superior separation efficiency and accuracy over common ultracentrifugation and ultrafiltration methods and displayed comparable or even better separation efficiency compared with size-exclusion chromatography, as demonstrated by the determination of encapsulation efficiency, Tyndall effect, transmittance, and drug recovery. DCC was also proven to minimally impact the size distribution, surface morphology, and thermal properties of the nanoparticles and liposomes, and moreover, it did not affect the determination of drug concentrations. Together, DCC has been demonstrated as a neat and effective method for the separation of free drugs from drug-loaded SLNs and liposomes, which shall be of ...Continue Reading

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Citations

Jul 25, 2021·Drug Delivery and Translational Research·Snežana ĐorđevićMaría J Vicent
Aug 8, 2021·Acta Biomaterialia·Natalia OnishchenkoElena Vodovozova

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Methods Mentioned

BETA
density gradient centrifugation

Software Mentioned

GraphPad Prism

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