A derivative of cationic antimicrobial protein attenuates lung injury by suppressing cell adhesion

American Journal of Respiratory Cell and Molecular Biology
S TasakaJ W Larrick

Abstract

Cationic antimicrobial protein of 18 kD (CAP18) was identified and purified from rabbit granulocytes and shown to inhibit various activities of lipopolysaccharide (LPS). We investigated the effect of a 32-amino-acid C-terminal fragment of CAP18 (CAP18-derived peptide, CDP) on the pathogenesis of acute lung injury caused by intravenous endotoxin. Guinea pigs were divided into six groups: (I) saline control (n = 8), (2) CDP-alone (n = 8), (3) LPS-alone (n = 8), (4) LPS+CDP0m (n = 8), (5) LPS+CDP10m (n = 8), and (6) LPS+CDP60m (n = 8). A CDP dose of 0.2 mg/kg was injected at various time points after LPS injection. Lung wet-to-dry weight ratio, [125I]albumin leakage in lung tissue and bronchoalveolar lavage (BAL) fluid, differential cell count in BAL fluid, and histopathologic features were examined 4 h after intravenous administration of 0.02 mg/kg of LPS. The LPS+CDP0m and the LPS+CDP10m groups showed significantly attenuated lung injury compared to that seen in the LPS-alone group, however the LPS+CDP60m group revealed no attenuation of lung injury. The accumulation of peripheral white blood cells into pulmonary vasculature was attenuated only in the LPS+CDP0m but not in the LPS+CDP10m groups. We examined the effect of CDP on t...Continue Reading

Citations

Jul 8, 2011·International Journal of Antimicrobial Agents·Nicole K Brogden, Kim A Brogden
Nov 19, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Fitzroy J ByfieldPaul A Janmey
Sep 28, 2017·American Journal of Respiratory Cell and Molecular Biology·Jordanna G JaynePaul M Beringer
Sep 16, 1998·Antimicrobial Agents and Chemotherapy·J TurnerR I Lehrer

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