A Designed Enzyme Promotes Selective Post-translational Acylation

Chembiochem : a European Journal of Chemical Biology
Pallavi M GosaviIvan V Korendovych

Abstract

A computationally designed, allosterically regulated catalyst (CaM M144H) produced by substituting a single residue in calmodulin, a non-enzymatic protein, is capable of efficient and site selective post-translational acylation of lysines in peptides with highly diverse sequences. Calmodulin's binding partners are involved in regulating a large number of cellular processes; this new chemical-biology tool will help to identify them and provide structural insight into their interactions with calmodulin.

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Citations

Jun 27, 2020·Chemistry : a European Journal·Kalani KariyawasamRémy Ricoux
Oct 4, 2019·ACS Catalysis·Liam R MarshallIvan V Korendovych

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