A detailed investigation of the porcine skin and nose microbiome using universal and Staphylococcus specific primers

Scientific Reports
Mikael Lenz StrubeKarl Pedersen

Abstract

MRSA is an increasing problem in humans as well as livestock. The bacterial co-colonization of the skin in MRSA carriers has been poorly investigated and moreover, there have been no methods for high resolution investigations of the Staphylococcus genus apart from tediously culturing or doing multiple PCRs. On 120 samples from pig ear, skin and nose, we generated amplicons from the V1-V2 region of the 16S rRNA gene to gather an overview of the genus-level microbiome, along with using MRSA specific plates to count MRSA. In parallel with this, amplicons of the tuf gene were generated, targeting only a region of the tuf gene found only in the Staphylococcus genus. Using these methods, we determined a core microbiota across the healthy pig and determined the Staphylococcus genus to be dominated by S. equorum. Moreover, we found Streptococcus to be inversely associated with Staphylococcus and MRSA, suggesting a role for this genus in combating MRSA. In this work, we have thoroughly investigated the skin and nose microbiome of the pig and developed a high throughput method for profiling the Staphylococcus genus which we believe will be useful for further investigations.

References

Jun 28, 2001·Journal of Clinical Microbiology·F MartineauM G Bergeron
Dec 23, 2004·Systematic and Applied Microbiology·Giuseppe BlaiottaFrancesco Villani
Dec 17, 2009·International Journal of Medical Microbiology : IJMM·Christiane CunyWolfgang Witte
Aug 17, 2010·Bioinformatics·Robert C Edgar
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
May 4, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E Petinaki, I Spiliopoulou
Dec 21, 2014·Applied and Environmental Microbiology·Mikael Lenz StrubeMette Boye
Sep 4, 2015·The Journal of Infection·Mary T BessesenDaniel N Frank
Sep 24, 2015·BMC Microbiology·Mackenzie Jonathan SlifierzJ Scott Weese
Nov 26, 2015·Science Advances·Cindy M LiuPaal Skytt Andersen
Aug 16, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Carl Andreas GrøntvedtJørgen Vildershøj Bjørnholt

❮ Previous
Next ❯

Citations

Jan 12, 2020·FEMS Microbiology Ecology·Connie A RojasKevin R Theis
Jan 15, 2019·Journal of Dairy Science·Bettina NonnemannKarl Pedersen
Dec 19, 2019·Frontiers in Microbiology·Julie E HansenKarl Pedersen
Aug 2, 2020·Environment International·Roosmarijn E C LuikenUNKNOWN EFFORT-group
Jan 28, 2021·Animal Microbiome·Mattia PiroloLuca Guardabassi
Jul 3, 2021·Antibiotics·Kirsi J AaltonenTarja Sironen
Jul 27, 2021·Advanced Materials Technologies·Jill ZiesmerGeorgios A Sotiriou
Jul 30, 2021·BMC Microbiology·Charlotte Marie AhleHolger Brüggemann
Jul 1, 2021·Applied and Environmental Microbiology·Anna Cäcilia InghamAnders Rhod Larsen

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
PCRs

Software Mentioned

ANOSIM
Adonis
USEARCH
agricolae
UCHIME
BION
blastn
BLAST
vegan
meta

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.