A Detailed Model of Electroenzymatic Glutamate Biosensors To Aid in Sensor Optimization and in Applications in Vivo

ACS Chemical Neuroscience
Mackenzie Clay, Harold G Monbouquette

Abstract

Simulations conducted with a detailed model of glutamate biosensor performance describe the observed sensor performance well, illustrate the limits of sensor performance, and suggest a path toward sensor optimization. Glutamate is the most important excitatory neurotransmitter in the brain, and electroenzymatic sensors have emerged as a useful tool for the monitoring of glutamate signaling in vivo. However, the utility of these sensors currently is limited by their sensitivity and response time. A mathematical model of a typical glutamate biosensor consisting of a Pt electrode coated with a permselective polymer film and a top layer of cross-linked glutamate oxidase has been constructed in terms of differential material balances on glutamate, H2O2, and O2 in one spatial dimension. Simulations suggest that reducing thicknesses of the permselective polymer and enzyme layers can increase sensitivity ∼6-fold and reduce response time ∼7-fold, and thereby improve resolution of transient glutamate signals. At currently employed enzyme layer thicknesses, both intrinsic enzyme kinetics and enzyme deactivation likely are masked by mass transfer. However, O2-dependence studies show essentially no reduction in signal at the lowest anticipa...Continue Reading

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Citations

Mar 11, 2020·Annual Review of Analytical Chemistry·Jacqueline D KeighronAnn-Sofie Cans
Feb 23, 2020·Journal of Neural Engineering·Jeeun KangDean F Wong
Jul 30, 2020·Analytical Methods : Advancing Methods and Applications·Yangguang OuParastoo Hashemi
Jan 31, 2020·The Analyst·I-Wen HuangHarold G Monbouquette
Dec 17, 2020·The Analyst·I-Wen HuangHarold G Monbouquette
Dec 24, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Yuanmo WangAnn-Sofie Cans
Feb 8, 2019·Biosensors & Bioelectronics·Mallikarjunarao GanesanaB Jill Venton
Apr 29, 2021·ACS Applied Materials & Interfaces·Joohee JeonTaek Dong Chung
Nov 14, 2019·ACS Sensors·Cong XuLanqun Mao
May 10, 2020·ACS Applied Materials & Interfaces·Yifei SunAlexander A Chubykin

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