A determining factor for insect feeding preference in the silkworm, Bombyx mori

PLoS Biology
Zhongjie ZhangAnjiang Tan

Abstract

Feeding preference is critical for insect adaptation and survival. However, little is known regarding the determination of insect feeding preference, and the genetic basis is poorly understood. As a model lepidopteran insect with economic importance, the domesticated silkworm, Bombyx mori, is a well-known monophagous insect that predominantly feeds on fresh mulberry leaves. This species-specific feeding preference provides an excellent model for investigation of host-plant selection of insects, although the molecular mechanism underlying this phenomenon remains unknown. Here, we describe the gene GR66, which encodes a putative bitter gustatory receptor (GR) that is responsible for the mulberry-specific feeding preference of B. mori. With the aid of a transposon-based, clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system, the GR66 locus was genetically mutated, and homozygous mutant silkworm strains with truncated gustatory receptor 66 (GR66) proteins were established. GR66 mutant larvae acquired new feeding activity, exhibiting the ability to feed on a number of plant species in addition to mulberry leaves, including fresh fruits and grain seeds that are not norma...Continue Reading

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Citations

Aug 25, 2020·Drug Discoveries & Therapeutics·Atmika PaudelKazuhisa Sekimizu
Jan 28, 2020·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Zhilei Zhao, Carolyn S McBride
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Feb 27, 2021·Bulletin of Entomological Research·Lu-Lu LiYa-Nan Zhang
Jul 16, 2021·PLoS Genetics·Noah K Whiteman, Julianne N Peláez
Jul 12, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Gen-Ceng LiNai-Yong Liu

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Methods Mentioned

BETA
targeted mutations
PCR
transfection
transgenic
fluorescence microscopy
genotyping
dissecting
reverse transcription-PCR

Software Mentioned

Autospike

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