A developmentally regulated wound epithelial antigen of the newt limb regenerate is also present in a variety of secretory/transport cell types

Developmental Biology
D J GoldhamerR A Tassava

Abstract

The role of the wound epithelium in amphibian limb regeneration is not understood. We showed previously that monoclonal antibody (mAb) WE3 stains the wound epithelium but not skin epidermis, suggesting that the WE3 antigen may be a marker for, or be important in, the function of the wound epithelium. In the present study, we conducted an extensive immunohistochemical survey of adult newt tissues to define the distribution of the WE3 antigen. The results show that the antigen is most commonly found in tissues specialized in macromolecular secretion and/or ion transport. Since the enzyme, carbonic anhydrase, serves as a useful marker for a variety of specialized transporting cell types, we examined whether this enzyme was present in WE3-reactive cells. Of the tissues examined, a striking degree of colocalization of carbonic anhydrase and the WE3 antigen was observed, further strengthening the view that the WE3 antigen is an important constituent of specialized transporting cells. A preliminary biochemical characterization suggests that the antigen is probably a glycoprotein, which elutes during gel filtration as a species of over 660 kDa. Possible implications for the function of the wound epithelium are discussed.

References

Jan 1, 1978·The Journal of Membrane Biology·C L Voûte, W Meier
Sep 1, 1977·The American Journal of Anatomy·N KrishnanM Singer
Sep 2, 1975·Differentiation; Research in Biological Diversity·R A Tassava, A L Mescher
Feb 1, 1988·Cell and Tissue Research·U Katz, S Gabbay
Jan 1, 1988·Archives of Biochemistry and Biophysics·S J Dodgson, L C Contino
Aug 1, 1987·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·R A TassavaB L Tomlinson
Aug 1, 1986·The Journal of Experimental Zoology·R A TassavaJ Gross
Jul 18, 1985·The New England Journal of Medicine·T H Maren
Apr 1, 1986·Experimental Cell Research·M T Campbell, J W McAvoy
Jan 1, 1967·Histochemie. Histochemistry. Histochimie·H P Hansson
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Jan 1, 1972·Tissue & Cell·R M Lavker
Jan 1, 1969·Histochemie. Histochemistry. Histochimie·S Yokota
Jan 1, 1969·Histochemie. Histochemistry. Histochimie·I Pesetsky
Jul 1, 1965·The Journal of Cell Biology·M G Farquhar, G E Palade
Jan 1, 1966·Journal of Morphology·A M Peadon, M Singer
Jan 1, 1984·Molecular and Cellular Biochemistry·J Kubilus, H P Baden
Jan 1, 1984·Annals of the New York Academy of Sciences·T Kumpulainen
Jan 1, 1984·Annals of the New York Academy of Sciences·S J DodgsonB T Storey
Jan 1, 1984·Annals of the New York Academy of Sciences·R M EffrosK Taki
Mar 1, 1957·The Journal of Experimental Zoology·C S THORNTON

❮ Previous
Next ❯

Citations

Jan 1, 2015·BMC Developmental Biology·Mariana G SimõesAntonio Jacinto
Mar 8, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·R N Christensen, R A Tassava
Jan 5, 2018·Regeneration·David L Stocum
Mar 1, 1991·The Journal of Experimental Zoology·H Onda, R A Tassava
Jul 4, 2018·Current Pathobiology Reports·Ricardo LondonoThomas P Lozito

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.