A diagnostic oligonucleotide microarray for simultaneous detection of grapevine viruses.

Journal of Virological Methods
Esteban A EngelPablo D T Valenzuela

Abstract

At least 58 viruses have been reported to infect grapevines causing economic damage globally. Conventional detection strategies based on serological assays, biological indexing and RT-PCR targeting one or few viruses in each assay are widely used. Grapevines are prone to contain mixed infections of several viruses, making the use of these techniques time-consuming. A 70-mer oligonucleotide microarray able to detect simultaneously a broad spectrum of known viruses as well as new viruses by cross-hybridization to highly conserved probes is reported in the present study. The array contains 570 unique probes designed against highly conserved and species-specific regions of 44 plant viral genomes. In addition probes designed against plant housekeeping genes are also included. By using a random primed RT-PCR amplification strategy of grapevine double stranded RNA-enriched samples, viral agents were detected in single and mixed infections. The microarray accuracy to detect 10 grapevine viruses was compared with RT-PCR yielding consistent results. For this purpose, grapevine samples containing single or mixed infections of Grapevine leafroll-associated virus-1, -2, -3, -4, -7, -9, Grapevine fanleaf virus, Grapevine rupestris stem pitti...Continue Reading

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Citations

Oct 30, 2012·The Journal of Microbiology·Ju-Mi KimGwangPyo Ko
Jul 23, 2011·Archives of Virology·Jacques du PreezJohan T Burger
May 15, 2012·Journal of Virological Methods·Jeremy R ThompsonKeith L Perry
May 18, 2010·Journal of Virological Methods·Antonio TiberiniAhmed Hadidi
Nov 26, 2011·Journal of Virological Methods·Fatima OsmanAdib Rowhani
Jul 20, 2010·Journal of Virological Methods·Bright O AgindotanCarl A Bradley
Jun 20, 2012·Journal of Virological Methods·Antonio Tiberini, Marina Barba
Aug 9, 2011·Journal of Virological Methods·Ismail AbdullahiMichael Rott
Jun 6, 2012·Journal of Virological Methods·Mitzi van der HeijdenLouis C Penning
Sep 8, 2015·Analytical Biochemistry·Ping WangYingwu Xu
Feb 8, 2011·Journal of Virological Methods·Mogens Nicolaisen
Sep 1, 2014·Plant Disease·Rayapati NaiduGiovanni P Martelli
Aug 9, 2020·Frontiers in Plant Science·Luis RubioInmaculada Ferriol
Sep 27, 2018·Frontiers in Microbiology·Vicente PallásDelano James
Feb 18, 2021·Talanta·Jiaqi BuShian Zhong

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Methods Mentioned

BETA
ELISA
scrapings
PCR

Software Mentioned

GenePix
Cluster
TreeView
BLASTN
ScanArray Express

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