PMID: 14415404Dec 1, 1959Paper

A diazo coupling method for the electron microscopic localization of cholinesterase

The Journal of Biophysical and Biochemical Cytology
G M LEHRER, L ORNSTEIN

Abstract

The presence of cholinesterase at the myoneural junction of intercostal muscle has been demonstrated in both light and electron microscopic preparations. A new simultaneous diazo coupling technique using alpha-naphthyl acetate as substrate and "hexazonium pararosanilin" as coupler has been applied to cold formalin-fixed tissues. After postfixation in buffered osmium tetroxide the sites of esterase activity are faithfully demonstrated at a high level of resolution. The details of cholin-esterase distribution and some technical aspects of the procedure are discussed.

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Citations

Jan 1, 1969·Histochemie. Histochemistry. Histochimie·H Teräväinen
Jan 1, 1987·Anatomy and Embryology·J Kucera, J M Walro
Jan 1, 1967·Histochemie. Histochemistry. Histochimie·R A BergmanA M Seligman
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Aug 8, 2002·Tissue & Cell·Marcia Miranda TorrejaisN A Basso
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Dec 1, 1961·The Journal of Biophysical and Biochemical Cytology·A WACHTELH GRUNDFEST
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Sep 1, 1971·Journal of Invertebrate Pathology·I D Bowen, D C Lloyd
Sep 1, 1962·Experimental Neurology·R M TORACK, R J BARRNETT

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