PMID: 2494645Mar 11, 1989Paper

A difference in the importance of bulged nucleotides and their parent base pairs in the binding of transcription factor IIIA to Xenopus 5S RNA and 5S RNA genes

Nucleic Acids Research
F Baudin, P J Romaniuk

Abstract

Individual bulge loops present in Xenopus 5S RNA (positions 49A-A50 in helix III, C63 in helix II and A83 in helix IV), were deleted by site directed mutagenesis. The interaction of these mutant 5S RNA molecules with TFIIIA was measured by a direct binding assay and a competition assay. The results of these experiments show that none of the bulged nucleotides in Xenopus 5S RNA are required for the binding of TFIIIA. The affinity of the mutant 5S RNA genes for TFIIIA was also studied by a filter binding assay. In contrast to the effect that deleting bulged nucleotides had on the TFIIIA-RNA binding affinity, deletion of the corresponding A-T base pair at position +83 in 5S DNA was found to reduce the apparent association constant of TFIIIA by a factor of four-fold.

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Citations

Mar 31, 2011·Biochemistry·J Benjamin Scripture, Paul W Huber
Nov 13, 2012·Biochimica Et Biophysica Acta·Elodie LayatSylvette Tourmente
May 15, 1990·Biochemistry·T P CallaciC W Wu
Feb 1, 1990·Nature·A BhattacharyyaD M Lilley
Jan 12, 1996·The Journal of Biological Chemistry·S L RawlingsP W Huber
Jan 1, 1991·Progress in Biophysics and Molecular Biology·B S Shastry
Jul 1, 1992·Molecular and Cellular Biology·M K Darby, K E Joho

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