A different view on DNA amplifications indicates frequent, highly complex, and stable amplicons on 12q13-21 in glioma

Molecular Cancer Research : MCR
Ulrike FischerEckart Meese

Abstract

To further understand the biological significance of amplifications for glioma development and recurrencies, we characterized amplicon frequency and size in low-grade glioma and amplicon stability in vivo in recurring glioblastoma. We developed a 12q13-21 amplicon-specific genomic microarray and a bioinformatics amplification prediction tool to analyze amplicon frequency, size, and maintenance in 40 glioma samples including 16 glioblastoma, 10 anaplastic astrocytoma, 7 astrocytoma WHO grade 2, and 7 pilocytic astrocytoma. Whereas previous studies reported two amplified subregions, we found a more complex situation with many amplified subregions. Analyzing 40 glioma, we found that all analyzed glioblastoma and the majority of pilocytic astrocytoma, grade 2 astrocytoma, and anaplastic astrocytoma showed at least one amplified subregion, indicating a much higher amplification frequency than previously suggested. Amplifications in low-grade glioma were smaller in size and displayed clearly different distribution patterns than amplifications in glioblastoma. One glioblastoma and its recurrencies revealed an amplified subregion of 5 Mb that was stable for 6 years. Expression analysis of the amplified region revealed 10 overexpressed ...Continue Reading

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Citations

Oct 1, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Wenjun ZhangLidija Pestic-Dragovich
Mar 24, 2009·Neuro-oncology·Helena NordTeresita Díaz de Ståhl
Sep 9, 2010·PLoS Computational Biology·Joseph E LucasJen-Tsan A Chi
Jun 23, 2011·PloS One·Andreas KellerEckart Meese
Nov 12, 2015·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Leo Y Luo, William C Hahn
Jun 24, 2010·Pathology, Research and Practice·Heidrun HollandRonald Koschny
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Dec 5, 2014·Nature Communications·Sergey NikolaevStylianos E Antonarakis
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May 28, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Ulrike FischerEckart Meese

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