A differential fluorescent receptor for nucleic acid analysis

Chembiochem : a European Journal of Chemical Biology
Hillary N Bengtson, Dmitry M Kolpashchikov

Abstract

Differential receptors use an array of sensors to recognize analytes. Each sensor in the array can recognize not one, but several analytes with different rates, so a single analyte triggers a response of several sensors in the array. The receptor thus produces a pattern of signals that is unique for each analyte, thereby enabling identification of a specific analyte by producing a "fingerprint" pattern. We applied this approach for the analysis of DNA sequences of Mycobacterium tuberculosis strains that differ by single nucleotide substitutions in the 81-bp hot-spot region that imparts rifampin resistance. The technology takes advantage of the new multicomponent, selfassembling sensor, which produces a fluorescent signal in the presence of specific DNA sequences. A differential fluorescent receptor (DFR) contained an array of three such sensors and differentiated at least eight DNA sequences. The approach requires only one molecular-beacon-like fluorescent reporter, which can be used by all three sensors. The DFR developed in this study represents a cost-efficient alternative to molecular diagnostic technologies that use fluorescent hybridization probes.

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Citations

Nov 3, 2016·Chembiochem : a European Journal of Chemical Biology·Amanda J CoxDmitry M Kolpashchikov
Jan 27, 2018·ChemistrySelect·Alexandra L Smith, Dmitry M Kolpashchikov
Jan 27, 2019·Analytical Chemistry·Nanami KikuchiDmitry M Kolpashchikov
Jun 28, 2019·Accounts of Chemical Research·Dmitry M Kolpashchikov

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