PMID: 6171054Jul 1, 1981Paper

A differential staining technique for simultaneous visualization of mitotic spindle and chromosomes in mammalian cells

Stain Technology
W L WissingerR J Wang

Abstract

A new technique has been devised for staining the mitotic spindle in mammalian cells while preserving spindle structure and chromosome number. The cells are trypsinized and fixed with a 3:1 methanol:acetic acid solution containing 4 mM MgCl2 and 1.5 mM CaCl2 at room temperature. The cells are then placed on slides and treated with 5% perchloric acid before staining with a 10% acetic acid solution containing safranin O and brilliant blue R. The preserved spindles appear dark blue against a light cytoplasmic background with chromosomes stained bright red. Individual chromosomes and chromatids are clearly visible. Positioning of the chromosomes relative to the spindle apparatus is readily ascertained allowing easy study of mitotic spindle and chromosome behavior.

Citations

Feb 1, 1987·Mutation Research·A R De Oliveira, G M Machado-Santelli
Apr 4, 1998·Microscopy Research and Technique·I TallonM Kirsch-Volders
Mar 31, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Zoé EliasBice Fubini
Aug 1, 1993·American Journal of Respiratory Cell and Molecular Biology·M YeglesM C Jaurand
Oct 27, 2020·Journal of Family Medicine and Primary Care·Richa BansalSonika Sharma

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