A DIGE proteomic analysis for high-intensity exercise-trained rat skeletal muscle

Journal of Biochemistry
Wataru YamaguchiIzumi Tabata

Abstract

Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. In this study, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after high-intensity exercise training (HIT). For 5 days, rats performed HIT, which consisted of 14 20-s swimming exercise bouts carrying a weight (14% of the body weight), and 10-s pause between bouts. The 2D-DIGE analysis was conducted on epitrochlearis muscles excised 18 h after the final training exercise. Proteomic profiling revealed that out of 800 detected and matched spots, 13 proteins exhibited changed expression by HIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Furthermore, using western immunoblot analyses, significantly changed expressions of NDUFS1 and parvalbumin (PV) were validated in relation to HIT. In conclusion, the proteomic 2D-DIGE analysis following HIT-identified expressions of NDUFS1 and PV, previously unknown to have functions related to exercise-training adaptations.

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Citations

Feb 8, 2013·Biomarkers in Medicine·Kay Ohlendieck
Mar 19, 2011·Journal of Proteomics·Cecilia GelfiPaolo Cerretelli
Dec 4, 2015·Proteomics·Ana Isabel PadrãoJosé Alberto Duarte
Apr 20, 2011·Journal of Cellular Physiology·Bernardo A PetrizOctávio L Franco
Sep 29, 2011·Proteomics·Agnese ViganòCecilia Gelfi
Jun 5, 2014·Proteomics. Clinical Applications·Heinrich Brinkmeier, Kay Ohlendieck
May 2, 2012·Journal of Proteomics·Fausto ScoppettaElisabetta Chiaradia
May 4, 2017·Medicine and Science in Sports and Exercise·Kaori MatsuoIzumi Tabata
Dec 8, 2017·Clinical and Experimental Pharmacology & Physiology·Liang ChenKeping Chen
Jun 21, 2012·Circulation. Cardiovascular Genetics·Shaan ChughAnthony O Gramolini
Feb 8, 2014·Journal of Applied Physiology·David P FergusonJ Timothy Lightfoot
Apr 21, 2019·The Journal of Physiological Sciences : JPS·Izumi Tabata
Jun 18, 2011·Expert Review of Proteomics·Jatin G Burniston, Eric P Hoffman
Jun 20, 2013·Expert Review of Proteomics·Ashling Holland, Kay Ohlendieck
Jun 12, 2012·Journal of Proteome Research·Miguel Martin-PerezJosefina Blasco

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