A digital protein microarray for COVID-19 cytokine storm monitoring.

Lab on a Chip
Yujing SongKatsuo Kurabayashi

Abstract

Despite widespread concern regarding cytokine storms leading to severe morbidity in COVID-19, rapid cytokine assays are not routinely available for monitoring critically ill patients. We report the clinical application of a digital protein microarray platform for rapid multiplex quantification of cytokines from critically ill COVID-19 patients admitted to the intensive care unit (ICU) at the University of Michigan Hospital. The platform comprises two low-cost modules: (i) a semi-automated fluidic dispensing/mixing module that can be operated inside a biosafety cabinet to minimize the exposure of the technician to the virus infection and (ii) a 12-12-15 inch compact fluorescence optical scanner for the potential near-bedside readout. The platform enabled daily cytokine analysis in clinical practice with high sensitivity (<0.4 pg mL-1), inter-assay repeatability (∼10% CV), and rapid operation providing feedback on the progress of therapy within 4 hours. This test allowed us to perform serial monitoring of two critically ill patients with respiratory failure and to support immunomodulatory therapy using the selective cytopheretic device (SCD). We also observed clear interleukin-6 (IL-6) elevations after receiving tocilizumab (IL-6...Continue Reading

References

Sep 27, 2015·Advanced Drug Delivery Reviews·Pengyu ChenKatsuo Kurabayashi
Jul 7, 2016·Accounts of Chemical Research·Michael P McRaeJohn T McDevitt
Dec 3, 2016·BMC Pulmonary Medicine·Rita de Cássia Coelho Moraes de BritoGiselia Alves Pontes da Silva
May 27, 2017·The Analyst·Xiaotian TanXudong Fan
Sep 20, 2017·Nature Reviews. Clinical Oncology·Sattva S NeelapuElizabeth J Shpall
Dec 2, 2017·Analytical Chemistry·Yi Zhang, Hiroyuki Noji
Feb 16, 2019·Proceedings of the National Academy of Sciences of the United States of America·Venkata YelleswarapuDavid Issadore
May 28, 2019·Biosensors & Bioelectronics·Jingjing SunYing Mu
Jun 21, 2019·Expert Review of Clinical Immunology·Chelsea KotchDavid T Teachey
Mar 4, 2020·Nature Microbiology·UNKNOWN Coronaviridae Study Group of the International Committee on Taxonomy of Viruses
Mar 10, 2020·American Journal of Respiratory and Critical Care Medicine·Matthew W SemlerLora A Reineck
Mar 21, 2020·Lancet·Puja MehtaUNKNOWN HLH Across Speciality Collaboration, UK
Apr 19, 2020·Science·John B Moore, Carl H June
Apr 23, 2020·Cell Host & Microbe·Evangelos J Giamarellos-BourboulisAntonia Koutsoukou
May 11, 2020·Best Practice & Research. Clinical Rheumatology·Georgia GriffinGrant C Hughes
Jul 1, 2020·JAMA Internal Medicine·Pratik SinhaCarolyn S Calfee
Jul 24, 2020·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Lenar YessayanDavid Humes
Aug 26, 2020·Nature Medicine·Diane Marie Del ValleSacha Gnjatic
Sep 4, 2020·JAMA : the Journal of the American Medical Association·Matthijs KoxPeter Pickkers
Oct 21, 2020·JAMA Internal Medicine·Shruti GuptaUNKNOWN STOP-COVID Investigators
Oct 21, 2020·JAMA Internal Medicine·Olivier HermineUNKNOWN CORIMUNO-19 Collaborative Group
Oct 22, 2020·The New England Journal of Medicine·John H StoneUNKNOWN BACC Bay Tocilizumab Trial Investigators
Nov 3, 2020·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Lenar YessayanH David Humes

❮ Previous
Next ❯

Citations

Mar 2, 2021·Biosensors & Bioelectronics·Yujing SongKatsuo Kurabayashi
Apr 4, 2021·Sensors·Suparshya Babu SukhavasiAbdelrahman Elleithy
Jun 11, 2021·Annals of Laboratory Medicine·Giulia BivonaMarcello Ciaccio
Jul 27, 2021·Frontiers in Oncology·Apriliana E R KartikasariMagdalena Plebanski

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
chip
fluorescence imaging
biosensor
biosensing
chips
Assay
ELISA
blood draws
blood draw

Software Mentioned

PEdELISA
Arduino
Conv2D
AutoCAD

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.