PMID: 8944642Nov 1, 1996Paper

A dihydropyridine-sensitive T-type Ca2+ current is the main Ca2+ current carrier in mouse primary spermatocytes

The American Journal of Physiology
C M SantiArturo Hernández-Cruz

Abstract

Ca2+ entry through Ca2+ channels is likely to play an important role in the differentiation of male germ cells as well as in fertilization by mature sperm. Here we present a detailed analysis of Ca2+ currents expressed in acutely dissociated mouse primary spermatocytes. Patch-clamp recordings demonstrated that the only voltage-gated Ca2+ channels present belong to the family of T-type Ca2+ currents. Accordingly, Ni2+ (200 microM) and amiloride (500 microM) reduced current amplitude by 75 and 62%, respectively. To our knowledge, this is the first report of a system where T-type Ca2+ channels are expressed in isolation. Unexpectedly, 5 and 10 microM nifedipine also reduced peak currents by 38 and 53%, respectively significant inhibition of the Ca2+ current occurred at concentrations as low as 2 microM. Because mature sperm cells are unable to synthesize new proteins, these Ca2+ channels are also likely to be present in these cells, where they may contribute to the Ca2+ influx required to trigger the acrosome reaction. This notion is supported by the fact that concentrations of Ni2+ and nifedipine, which block these Ca2+ currents, also inhibit the acrosome reaction. Because these channels represent the primary pathway for voltage-...Continue Reading

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Citations

May 1, 1998·Zygote : the Biology of Gametes and Early Embryos·O ZapataA Darszon
Nov 14, 2000·The Journal of Biological Chemistry·J E McRoryT P Snutch
Jun 13, 2002·Development, Growth & Differentiation·Weon-Young SonYoung-Kug Choo
Sep 10, 2005·Journal of Receptor and Signal Transduction Research·Ricardo Felix
Jan 13, 2006·The Journal of Biological Chemistry·Enrique O Hernández-GonzálezPablo E Visconti
Sep 26, 2007·Clinical and Experimental Pharmacology & Physiology·Seung-Kwon Yang, Chen Chen
Sep 23, 2010·Journal of Assisted Reproduction and Genetics·Jae Ho LeeChurl K Min
Oct 25, 2011·Annual Review of Physiology·Polina V LishkoDavid E Clapham
May 16, 2017·Journal of Morphology·Amin GolpourHamid Niksirat
Apr 10, 1999·Nature Neuroscience·R S ChuangK J Swartz
May 3, 2000·The Journal of Biological Chemistry·G WennemuthD F Babcock
Dec 31, 2002·Physiological Reviews·Edward Perez-Reyes
May 5, 2011·Asian Journal of Andrology·Pablo E ViscontiAlberto Darszon
Sep 8, 2011·Journal of Cellular Physiology·Enrique BalderasAlberto Darszon
Aug 1, 2013·Physiological Reviews·Eunji Cheong, Hee-Sup Shin
Mar 7, 2014·Pflügers Archiv : European journal of physiology·Alberto Darszon, Arturo Hernández-Cruz
Oct 1, 2016·Oncotarget·Shi-Kai Jin, Wan-Xi Yang
Mar 7, 1998·The Journal of Biological Chemistry·M SchreiberL Salkoff
Dec 13, 2005·Human Reproduction Update·C Jimenez-GonzalezS J Publicover
Jan 8, 2009·The Journal of Membrane Biology·Liang LuHang Xiao
Nov 23, 2005·The Journal of Biological Chemistry·Durga Prasad MishraChandrima Shaha
Oct 26, 2012·The Journal of Biological Chemistry·Jose Luis De La Vega-BeltranAlberto Darszon
Jul 30, 2015·Journal of Cellular Physiology·Ignacio López-GonzálezAlberto Darszon
Aug 22, 2001·FEBS Letters·D SánchezA Darszon
Mar 1, 2007·American Journal of Physiology. Endocrinology and Metabolism·Seung-Kwon YangChen Chen
Nov 3, 2010·Asian Journal of Andrology·Satish K Gupta, Beena Bhandari
Apr 30, 1999·Physiological Reviews·A DarszonF Espinosa
Nov 22, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Amin GolpourHamid Niksirat
Oct 31, 2019·Human Reproduction Update·Sean G BrownSarah J Martins da Silva

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