A direct and rapid leaf water extraction method for isotopic analysis

Rapid Communications in Mass Spectrometry : RCM
Leon I Peters, Dan Yakir

Abstract

Isotopic measurements of leaf water have provided insights into a range of ecophysiological and biogeochemical processes, but require an extraction step which often constitutes the major analytical bottleneck in large-scale studies. Current standard procedures for leaf water analysis are based on cryogenic vacuum or azeotrophic distillation, and are laborious, require sophisticated distillation lines and the use of toxic materials. We report a rapid technique based on centrifugation/filtration of leaf samples pulverised in their original sampling tubes, using a specifically adapted, simple apparatus. The leaf water extracts produced are suitable for isotopic analysis via pyrolysis gas chromatography isotope ratio mass spectrometry (PYR/GC/IRMS). The new method was validated against cryogenic vacuum distillation and showed an overall accuracy of +/-0.5 per thousand (nine grouped comparisons, n = 110) over a range of 21 per thousand. Effects due to the presence of soluble carbohydrates were near the detection limits for most samples analysed, and these effects could be corrected for (the extracted soluble organics could also be used for isotopic analysis). The extraction time for a routine eight-sample subset was reduced from 4 h...Continue Reading

References

Jun 14, 2003·Plant, Cell & Environment·G. D. Farquhar, K. S. Gan
May 1, 1992·Plant Physiology·Y H Luo, L Sternberg
Dec 13, 2006·Plant Physiology·Jirí SantrucekLenka Bulícková
May 1, 2000·Oecologia·D Yakir, L da S L Sternberg

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Citations

Dec 17, 2011·Rapid Communications in Mass Spectrometry : RCM·Markus SchmidtUlli Seibt
Oct 31, 2012·The New Phytologist·Verena PfahlerEmmanuel Frossard
May 6, 2016·The New Phytologist·Xin Song, Margaret M Barbour
Nov 20, 2016·Rapid Communications in Mass Spectrometry : RCM·M GajT Himmelsbach
Apr 13, 2018·Rapid Communications in Mass Spectrometry : RCM·Cody MillarJeffrey J McDonnell
Jul 16, 2019·Rapid Communications in Mass Spectrometry : RCM·Cody MillarJeffrey J McDonnell
Dec 16, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yongle ChenXin Song

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