A direct and rapid method to determine cyanide in urine by capillary electrophoresis

Journal of Chromatography. a
Qiyang ZhangMaojun Gong

Abstract

Cyanides are poisonous chemicals that widely exist in nature and industrial processes as well as accidental fires. Rapid and accurate determination of cyanide exposure would facilitate forensic investigation, medical diagnosis, and chronic cyanide monitoring. Here, a rapid and direct method was developed for the determination of cyanide ions in urinary samples. This technique was based on an integrated capillary electrophoresis system coupled with laser-induced fluorescence (LIF) detection. Cyanide ions were derivatized with naphthalene-2,3-dicarboxaldehyde (NDA) and a primary amine (glycine) for LIF detection. Three separate reagents, NDA, glycine, and cyanide sample, were mixed online, which secured uniform conditions between samples for cyanide derivatization and reduced the risk of precipitation formation of mixtures. Conditions were optimized; the derivatization was completed in 2-4min, and the separation was observed in 25s. The limit of detection (LOD) was 4.0nM at 3-fold signal-to-noise ratio for standard cyanide in buffer. The cyanide levels in urine samples from smokers and non-smokers were determined by using the method of standard addition, which demonstrated significant difference of cyanide levels in urinary sampl...Continue Reading

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Citations

Aug 2, 2017·Electrophoresis·Alain Wuethrich, Joselito P Quirino
May 18, 2017·Environmental Science and Pollution Research International·Ewa JaszczakJacek Namieśnik
Mar 1, 2020·Analytical and Bioanalytical Chemistry·Penghui LiLiangbao Yang
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ewa Jaszczak-WilkeŻaneta Polkowska

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