A direct comparison between extracted tooth and filter-membrane biofilm models of endodontic irrigation using Enterococcus faecalis

Archives of Microbiology
C K HopePeter J Farrelly

Abstract

Endodontic restorations often fail due to inadequate disinfection of the root canal even though the antimicrobial irrigants used have been shown to be capable of killing the bacterium frequently implicated in this complication, Enterococcus faecalis (Ef). Extracted human teeth were root-prepared and filled with a liquid culture of Ef. Following incubation, the root canals were irrigated with 1% sodium hypochlorite (NaOCl), electrochemically activated water or saline control. Irrigation was modelled using an electronic pipette to deliver the solutions at a reproducible flow velocity. A series of parallel experiments employed a membrane biofilm model that was directly immersed into irrigant. Experimental conditions where contiguous between the extracted tooth model and biofilm model wherever possible. After 60 s of exposure, 1% NaOCl effectively sterilised the biofilm model, whereas log 3.36 viable Ef where recoverable from the analogous extracted tooth model, the other irrigants proved ineffective. Biofilms of Ef were susceptible to concentrations of irrigant that proved ineffective in the tooth model. NaOCl was the most effective biocide in either case. This suggests that the biofilm modality of bacterial growth may not be the ...Continue Reading

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Citations

Aug 3, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R M S ThornD M Reynolds
Oct 12, 2011·International Endodontic Journal·R Ordinola-ZapataM A H Duarte
Jul 25, 2015·IEEE Transactions on Bio-medical Engineering·Alexandra GarraudDavid P Arnold
Dec 28, 2017·Journal of Conservative Dentistry : JCD·Sheetal Basavraj GhivariMadhu A Pujar
Nov 27, 2019·Journal of Clinical Medicine·Anna-Lena BöhmTanja Grobecker-Karl
Dec 10, 2019·Current Microbiology·Simonetta D'ErcoleDomenico Tripodi
Nov 1, 2013·The Journal of Contemporary Dental Practice·Resmiye Ebru TiraliAtilla Elhan

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