A direct comparison of approaches for increasing carbon flow to aromatic biosynthesis in Escherichia coli

Journal of Industrial Microbiology
Guillermo GossetA Berry

Abstract

Different approaches to increasing carbon commitment to aromatic amino acid biosynthesis were compared in isogenic strains of Escherichia coli. In a strain having a wild-type PEP:glucose phosphotransferase (PTS) system, inactivation of the genes encoding pyruvate kinase (pykA and pykF) resulted in a 3.4 fold increase in carbon flow to aromatic biosynthesis. In a strain already having increased carbon flow to aromatics by virtue of overexpression of the tktA gene (encoding transketolase), the pykA and/or pykf mutations had no effect. A PTS- glucose+ mutant showed a 1.6-fold increase in carbon flow to aromatics compared to the PTS+ control strain. In the PTS- glucose+ host background, overexpression of tktA caused a further 3.7-fold increase in carbon flow, while inactivation of pykA and pykF caused a 5.8-fold increase. When all of the variables tested (PTS-glucose+, pykA, pykF, and overexpressed tktA) were combined in a single strain, a 19.9-fold increase in carbon commitment to aromatic biosynthesis was achieved.

References

Dec 1, 1992·Journal of Bacteriology·R PatnaikJ C Liao
Jul 1, 1990·Bio/technology·G della-CioppaL K Grill
Oct 1, 1991·Applied and Environmental Microbiology·D MascarenhasC S Chen
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A R WymanD Botstein

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Citations

Dec 24, 2005·Applied Microbiology and Biotechnology·Masato Ikeda
Apr 1, 1997·Current Opinion in Biotechnology·D C Cameron, F W Chaplen
Jan 30, 1999·The International Journal of Biochemistry & Cell Biology·G SchenkP F Nixon
Apr 12, 2011·Applied and Environmental Microbiology·Steffen N LindnerVolker F Wendisch
Nov 5, 2005·Applied and Environmental Microbiology·T ZhuM M Domach
Nov 15, 2013·Microbial Cell Factories·María I Chávez-BéjarGuillermo Gosset
Sep 21, 2007·Microbial Cell Factories·José Luis Báez-ViverosGuillermo Gosset
Apr 4, 2009·Microbial Cell Factories·Víctor E Balderas-HernándezGuillermo Gosset
Jun 6, 2014·PLoS Computational Biology·Steven W SowaLydia M Contreras
Sep 5, 2009·PloS One·Matthew L Rizk, James C Liao
Apr 23, 2011·Journal of Industrial Microbiology & Biotechnology·Ana Joyce MuñozGuillermo Gosset
Jul 5, 2011·Metabolic Engineering·Rebekah McKenna, David R Nielsen
Aug 17, 2005·FEMS Microbiology Reviews·Uwe Sauer, Bernhard J Eikmanns
Mar 12, 2015·Applied Microbiology and Biotechnology·Liangtian MiaoYanhe Ma
Apr 23, 2014·Biotechnology Advances·Neng-Zhong XiePing Xu
Jun 27, 2007·Biotechnology and Bioengineering·Christophe ChassagnoleMatthias Reuss
Sep 13, 2015·Applied and Environmental Microbiology·Sudeshna SenguptaVeeresh Juturu
Sep 11, 2018·Biotechnology and Bioengineering·Julia TröndleDirk Weuster-Botz
Dec 12, 2007·Biotechnology and Bioengineering·Ranjan PatnaikDavid F Kraynie
Sep 24, 2005·Journal of Molecular Microbiology and Biotechnology·Noemí FloresFrancisco Bolívar
Mar 12, 2013·Chemical Society Reviews·Manfred SchreweAndreas Schmid
Jan 18, 2003·World Journal of Gastroenterology : WJG·Yong-Qing WuWei-Da Huang
Apr 16, 2021·World Journal of Microbiology & Biotechnology·Liwen LiuPengfei Gu
Jan 11, 2005·Journal of Biotechnology·T PolenV F Wendisch

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