A direct dopaminergic control of aromatase activity in the quail preoptic area

The Journal of Steroid Biochemistry and Molecular Biology
M Baillien, Jacques Balthazart

Abstract

In the quail preoptic area (POA) anatomical and pharmacological data suggest that catecholamines may be implicated in the control of testosterone (T) aromatization into estrogens. The biochemical mechanism(s) mediating this control of the enzyme activity is (are) however unexplored. The present studies were carried out to investigate whether the catecholamines, dopamine (DA) and norepinephrine (NE) are able to directly affect aromatase activity (AA) measured during in vitro incubations of POA homogenates. AA was quantified in the POA-hypothalamus of adult male Japanese quail by measuring the tritiated water production from [1beta-3H]-androstenedione. Enzyme activity was linear as a function of the incubation time and of the protein content of homogenates. It exhibited a typical Michaelis-Menten kinetics, with an apparent Km of 2.8 nM and a Vmax of 266.6 fmol h(-1) mg wet weight(-1). AA was then measured at a substrate concentration of 25 nM in the presence of catecholamines and some of their receptor agonists or antagonists, at two concentrations, 10(-3) and 10(-6) M. Norepinephrine and prazosin (alpha1-adrenergic antagonist) had no or very limited effects on AA at both concentrations. In contrast, DA and some D1 and/or D2 rece...Continue Reading

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Citations

Jul 6, 2000·The Journal of Comparative Neurology·H EvrardJ Balthazart
Mar 31, 2009·Cellular and Molecular Neurobiology·Lubica KubíkováLubor Kostál
Jun 7, 2005·Behavioural Brain Research·Charlotte A CornilJacques Balthazart
Dec 18, 2001·Brain Research. Brain Research Reviews·P AbsilJ Balthazart
Jun 26, 1998·Trends in Neurosciences·J Balthazart, G F Ball
Feb 1, 2000·Trends in Neurosciences·I Balthazart, G F Ball
Apr 21, 1999·Brain Research Bulletin·M BaillienJ Balthazart
Feb 19, 2002·The Journal of Steroid Biochemistry and Molecular Biology·J BalthazartG F Ball
Nov 19, 2003·The Journal of Steroid Biochemistry and Molecular Biology·Jacques BalthazartGregory F Ball
May 9, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jacques BalthazartGregory F Ball
Feb 12, 2010·Neuroreport·Sylvia M BardetJacques Balthazart
May 12, 2011·PloS One·Charlotte A CornilThierry D Charlier
Aug 27, 2014·General and Comparative Endocrinology·Charlotte A CornilJacques Balthazart
Jun 10, 2009·Frontiers in Neuroendocrinology·Jean Luc Do RegoHubert Vaudry
May 16, 2009·Frontiers in Neuroendocrinology·Sarah E LondonBarney A Schlinger
Dec 29, 2015·Journal of Neurochemistry·Devon ComitoBarney A Schlinger
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Sep 25, 2012·Psychoneuroendocrinology·Catherine de BournonvilleCharlotte A Cornil
Apr 9, 2015·Frontiers in Neuroscience·Thierry D CharlierJacques Balthazart
Oct 30, 2014·Frontiers in Neuroscience·Takayoshi Ubuka, Kazuyoshi Tsutsui
May 18, 2016·Hormones and Behavior·Catherine de BournonvilleCharlotte A Cornil
Jul 28, 2016·BMC Complementary and Alternative Medicine·Steeve GressGilles-Éric Séralini

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