A direct observation of bacterial coverage and biofilm formation by Acidithiobacillus ferrooxidans on chalcopyrite and pyrite surfaces

Biofouling
Y YangM Chen

Abstract

To obtain a fundamental understanding of the population behaviour of Acidithiobacillus ferrooxidans at chalcopyrite and pyrite surfaces, the early stage attachment behaviour and biofilm formation by this bacterium on chalcopyrite (CuFeS2) and pyrite (FeS2) were studied by optical microscopy, Raman spectroscopy, time-of-flight secondary ion mass spectrometry (ToF-SIMS) and electron backscatter diffraction (EBSD). The results indicate there was no significant difference in selectivity of bacterial attachment between chalcopyrite and pyrite. However, the result of ToF-SIMS analysis suggests that the surface of the pyrite was covered more extensively by biofilm than that of the chalcopyrite, which may indicate more extracellular polymeric substances (EPS) formation by bacterial cells growing on pyrite. EBSD and optical image analysis indicated that selectivity of bacterial attachment to chalcopyrite was not significantly affected by crystal orientation. The results also suggest that the bacterial population in defective areas of chalcopyrite was significantly higher than on the polished surfaces.

References

Jul 2, 1998·Applied and Environmental Microbiology·T GehrkeW Sand
Jul 27, 2002·Annual Review of Microbiology·Douglas E Rawlings
Apr 1, 1959·The Journal of Pathology and Bacteriology·C A ROSS
Oct 27, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Stanley L ErlandsenCarol L Wells
May 31, 2013·Applied Microbiology and Biotechnology·Mario VeraWolfgang Sand

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Citations

Jun 23, 2016·Canadian Journal of Microbiology·Ayse Tuba KocamanKatrina Jane Edwards
Aug 20, 2017·Biointerphases·Wenchao WeiXiao-Ying Yu
Jul 9, 2021·International Journal of Systematic and Evolutionary Microbiology·Ruiyong ZhangAxel Schippers

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Methods Mentioned

BETA
optical microscopy
electron backscattered diffraction
scanning electron microscopy

Software Mentioned

Image J
WincadenceN
Bruker Esprit

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