A Direct Plasma miRNA Assay for Early Detection and Histological Classification of Lung Cancer

Translational Oncology
Qixin LengFeng Jiang

Abstract

Cell-free microRNAs in plasma provide circulating biomarkers for lung cancer. Most techniques for analysis of miRNAs require a large plasma volume to purify a sufficient RNA yield followed by complicated downstream processing. Small differences in the multiple procedures often cause large analytical variations and poor diagnostic values of the plasma biomarkers. Here we investigate whether directly quantifying plasma miRNAs without RNA purification could diagnose lung cancer. FirePlex assay was directly applied to 20 μl plasma of 56 lung cancer patients and 28 cancer free controls for quantifying 11 lung tumor-associated miRNAs. FirePlex assay is easier, less expensive and time-consuming for quantification of plasma miRNAs compared with conventional reverse transcription PCR with an equivalent analytic performance. From the lung tumor-associated miRNAs, a prediction model based on two miRNAs (miRs-205-5p and -210-3p) was developed, producing 78.6% sensitivity and 89.3% specificity for identifying lung cancer. The diagnostic value was independent of stage of lung tumor, and patients' age and sex (all P > 0.05). Furthermore, based on the same two miRNAs, additional prediction models were developed with 75.0% sensitivity and 89.3%...Continue Reading

Citations

Nov 2, 2019·Biomarkers in Medicine·Jin LiYang Ning
Sep 15, 2020·SLAS Discovery·Michael PlatchekWensheng Xie
Mar 19, 2020·Frontiers in Cell and Developmental Biology·Monica NeaguSabina Zurac
Jan 29, 2021·International Journal of Molecular Sciences·Francesca PrecazziniPaola Campomenosi
Dec 1, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Shen ZhongJürgen Borlak

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Methods Mentioned

BETA
PCR
Assay
flow cytometry
biopsy

Software Mentioned

LASSO
[UNK] Analysis Workbench
FirePlex

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