A direct test of the reductionist approach to structural studies of calmodulin activity: relevance of peptide models of target proteins

The Journal of Biological Chemistry
James K KranzA Joshua Wand

Abstract

Ca(2+)-saturated calmodulin (CaM) directly associates with and activates CaM-dependent protein kinase I (CaMKI) through interactions with a short sequence in its regulatory domain. Using heteronuclear NMR (13)C-(15)N-(1)H correlation experiments, the backbone assignments were determined for CaM bound to a peptide (CaMKIp) corresponding to the CaM-binding sequence of CaMKI. A comparison of chemical shifts for free CaM with those of the CaM. CaMKIp complex indicate large differences throughout the CaM sequence. Using NMR techniques optimized for large proteins, backbone resonance assignments were also determined for CaM bound to the intact CaMKI enzyme. NMR spectra of CaM bound to either the CaMKI enzyme or peptide are virtually identical, indicating that calmodulin is structurally indistinguishable when complexed to the intact kinase or the peptide CaM-binding domain. Chemical shifts of CaM bound to a peptide (smMLCKp) corresponding to the calmodulin-binding domain of smooth muscle myosin light chain kinase are also compared with the CaM. CaMKI complexes. Chemical shifts can differentiate one complex from another, as well as bound versus free states of CaM. In this context, the observed similarity between CaM. CaMKI enzyme and p...Continue Reading

References

Feb 1, 1990·Trends in Biochemical Sciences·K T O'Neil, W F DeGrado
May 2, 1985·Nature·Y S BabuW J Cook
Sep 1, 1995·Nature Structural Biology·H KuboniwaA Bax
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·A Crivici, M Ikura
Jan 1, 1995·Trends in Biochemical Sciences·P JamesE Carafoli
Sep 1, 1995·Journal of Biomolecular NMR·D S WishartB D Sykes
Apr 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A R Rhoads, F Friedberg
Jun 15, 1999·Trends in Biochemical Sciences·T R Soderling
Feb 8, 2000·The Journal of Biological Chemistry·A PersechiniP M Stemmer
Jul 8, 2000·Trends in Cell Biology·D Chin, A R Means
Mar 27, 2001·Protein Science : a Publication of the Protein Society·G LarssonS S Wijmenga
Jan 25, 2002·Nature·Robert C Liddington
Feb 20, 2002·Biochemistry·James K KranzA Joshua Wand

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Citations

Mar 2, 2011·Journal of Biomolecular NMR·Jessica L GiffordHans J Vogel
Apr 13, 2010·Nature Chemical Biology·Michael S MarlowA Joshua Wand
Jan 14, 2010·Molecular & Cellular Proteomics : MCP·David J O'ConnellDolores J Cahill
May 11, 2012·Journal of Biomolecular Structure & Dynamics·Krzysztof Kuczera, Petri Kursula
Aug 11, 2012·Journal of Biomolecular Structure & Dynamics·Deli IreneChia-Lin Chyan
Jun 20, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tanmay S ChavanVadim Gaponenko
May 15, 2012·Structure·Miao ZhangJi-fang Zhang
Jan 24, 2006·Biophysical Journal·Ingemar AndréSara Linse
Sep 4, 2004·Biophysical Journal·Ingemar AndréSara Linse
Aug 31, 2002·Biophysical Journal·Andriyka L PapishHans J Vogel
Sep 22, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chia-Lin ChyanSin-Mao Lin
Jan 19, 2018·Annual Review of Biophysics·A Joshua Wand, Kim A Sharp
Dec 8, 2004·The Journal of Biological Chemistry·Liangwen XiongSusan L Hamilton
Sep 8, 2006·The FEBS Journal·Juan Martinez-SanzConstantin T Craescu
Feb 3, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Sunmi KangSunghyouk Park
Jul 20, 2007·Nature·Kendra King FrederickA Joshua Wand
Jul 31, 2002·The Journal of Biological Chemistry·Alex DvoretskyPaul R Rosevear
Sep 29, 2007·The Journal of Biological Chemistry·Yubin ZhouJenny J Yang
Jun 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·José A CaroA Joshua Wand
Jul 10, 2016·Journal of Biomolecular NMR·Matthew A Stetz, A Joshua Wand
May 4, 2017·Proceedings of the National Academy of Sciences of the United States of America·Fei LiuJin Wang
Jun 10, 2008·Biochimica Et Biophysica Acta·Jinho KangSunghyouk Park

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