Neural tissue formation is induced by growth factors that activate networks of signal transduction cascades that ultimately lead to the expression of early neural genes, including transcription factors of the SoxB family. Here, we report that fibroblast growth factor (FGF)-induced Erk1/2 (Mapk3 and Mapk1, respectively) mitogen-activated protein kinase (MAPK), but not phosphatidylinositol 3'-OH kinase (PI3K, Pik3r1), signalling is required for neural specification in mouse embryonic stem (ES) cells and in the chick embryo. Further, blocking Erk1/2 inhibits the onset of key SoxB genes in both mouse ES cells (Sox1) and chick embryos (Sox2 and Sox3) and, in both contexts, Erk1/2 signalling is required during only a narrow time window, as neural specification takes place. In the absence of Erk1/2 signalling, differentiation of ES cells stalls following Fgf5 upregulation. Using differentiating ES cells as a model for neural specification, we demonstrate that sustained Erk1/2 activation controls the transition from an Fgf5-positive, primitive ectoderm-like cell state to a neural progenitor cell state without attenuating bone morphogenetic protein (BMP) signalling and we also define the minimum period of Erk1/2 activity required to med...Continue Reading
LIF-independent JAK signalling to chromatin in embryonic stem cells uncovered from an adult stem cell disease
JAK/STAT3 signalling is sufficient and dominant over antagonistic cues for the establishment of naive pluripotency
Geminin promotes neural fate acquisition of embryonic stem cells by maintaining chromatin in an accessible and hyperacetylated state
Attenuation of extrinsic signaling reveals the importance of matrix remodeling on maintenance of embryonic stem cell self-renewal
Selecting antagonistic antibodies that control differentiation through inducible expression in embryonic stem cells
Undersulfation of heparan sulfate restricts differentiation potential of mouse embryonic stem cells.
Immobilization of heparan sulfate on electrospun meshes to support embryonic stem cell culture and differentiation.
Unique and overlapping functions of GSK-3 isoforms in cell differentiation and proliferation and cardiovascular development
CD133 expression by neural progenitors derived from human embryonic stem cells and its use for their prospective isolation
Exacerbation of oxidative stress-induced cell death and differentiation in induced pluripotent stem cells lacking heme oxygenase-1
Neur-ons and neur-offs: regulators of neural induction in vertebrate embryos and embryonic stem cells
Mitogen-activated protein kinase signaling in the heart: angels versus demons in a heart-breaking tale
The response of early neural genes to FGF signaling or inhibition of BMP indicate the absence of a conserved neural induction module
FGF dependent regulation of Zfhx1b gene expression promotes the formation of definitive neural stem cells in the mouse anterior neurectoderm
Probing the role of stochasticity in a model of the embryonic stem cell: heterogeneous gene expression and reprogramming efficiency
Retinoic acid orchestrates fibroblast growth factor signalling to drive embryonic stem cell differentiation
Opposing Fgf and Bmp activities regulate the specification of olfactory sensory and respiratory epithelial cell fates
Temporally controlled modulation of FGF/ERK signaling directs midbrain dopaminergic neural progenitor fate in mouse and human pluripotent stem cells
Conversion from mouse embryonic to extra-embryonic endoderm stem cells reveals distinct differentiation capacities of pluripotent stem cell states
FGF4 is required for lineage restriction and salt-and-pepper distribution of primitive endoderm factors but not their initial expression in the mouse
A membrane-associated β-catenin/Oct4 complex correlates with ground-state pluripotency in mouse embryonic stem cells
Distinct roles for isoforms of the catalytic subunit of class-IA PI3K in the regulation of behaviour of murine embryonic stem cells
Functional heterogeneity of embryonic stem cells revealed through translational amplification of an early endodermal transcript
A genome-wide RNAi screen reveals MAP kinase phosphatases as key ERK pathway regulators during embryonic stem cell differentiation
A requirement for FGF signalling in the formation of primitive streak-like intermediates from primitive ectoderm in culture
Fgf8-related secondary organizers exert different polarizing planar instructions along the mouse anterior neural tube
Generation and characterization of a novel mouse embryonic stem cell line with a dynamic reporter of Nanog expression
MicroRNA-200a regulates Grb2 and suppresses differentiation of mouse embryonic stem cells into endoderm and mesoderm
Role of heme oxygenase-1 in postnatal differentiation of stem cells: a possible cross-talk with microRNAs
Dual functions of hypoxia-inducible factor 1 alpha for the commitment of mouse embryonic stem cells toward a neural lineage
Functional diversity of ESC-derived motor neuron subtypes revealed through intraspinal transplantation
Impaired neural differentiation potency by retinoic acid receptor-α pathway defect in induced pluripotent stem cells
BMP4 Signaling Acts via dual-specificity phosphatase 9 to control ERK activity in mouse embryonic stem cells
Lessons from the embryonic neural stem cell niche for neural lineage differentiation of pluripotent stem cells.
MAPK/ERK1/2 signaling mediates endothelial-like differentiation of immature DCs in the microenvironment of esophageal squamous cell carcinoma
Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ESC differentiation without affecting naïve to primed cell transition
Nucleostemin maintains self-renewal of embryonic stem cells and promotes reprogramming of somatic cells to pluripotency
FGF and retinoic acid activity gradients control the timing of neural crest cell emigration in the trunk
TAK1 promotes BMP4/Smad1 signaling via inhibition of erk MAPK: a new link in the FGF/BMP regulatory network
Glycocalyx remodeling with proteoglycan mimetics promotes neural specification in embryonic stem cells
SH2B1beta enhances fibroblast growth factor 1 (FGF1)-induced neurite outgrowth through MEK-ERK1/2-STAT3-Egr1 pathway
Maintenance of Self-Renewal and Pluripotency in J1 Mouse Embryonic Stem Cells through Regulating Transcription Factor and MicroRNA Expression Induced by PD0325901
Efficient derivation of dopaminergic neurons from SOX1⁻ floor plate cells under defined culture conditions
MAPK/ERK signalling mediates VEGF-induced bone marrow stem cell differentiation into endothelial cell
VEGF-A-induced immature DCs not mature DCs differentiation into endothelial-like cells through ERK1/2-dependent pathway
Mu and kappa opioids modulate mouse embryonic stem cell-derived neural progenitor differentiation via MAP kinases
FGF signalling as a mediator of lineage transitions--evidence from embryonic stem cell differentiation
Inhibition of ERK1/2 prevents neural and mesendodermal differentiation and promotes human embryonic stem cell self-renewal
Controlling embryonic stem cell proliferation and pluripotency: the role of PI3K- and GSK-3-dependent signalling
FGF signaling via MAPK is required early and improves Activin A-induced definitive endoderm formation from human embryonic stem cells
Activation of FGFR(IIIc) isoforms promotes activin-induced mesendoderm development in mouse embryonic stem cells and reduces Sox17 coexpression in EpCAM+ cells
Dissecting mechanisms of mouse embryonic stem cells heterogeneity through a model-based analysis of transcription factor dynamics
Pausing of RNA polymerase II regulates mammalian developmental potential through control of signaling networks
Tracing the derivation of embryonic stem cells from the inner cell mass by single-cell RNA-Seq analysis
VEGFR-1 mediates endothelial differentiation and formation of blood vessels in a murine model of infantile hemangioma
Independent regulation of Sox3 and Lmx1b by FGF and BMP signaling influences the neurogenic and non-neurogenic domains in the chick otic placode
Activated astrocytes enhance the dopaminergic differentiation of stem cells and promote brain repair through bFGF
Integration of signals along orthogonal axes of the vertebrate neural tube controls progenitor competence and increases cell diversity
Neural stem cells secrete factors facilitating brain regeneration upon constitutive Raf-Erk activation
In vitro culture of stem-like cells derived from somatic cell nuclear transfer bovine embryos of the Korean beef cattle species, HanWoo
Src Family Kinases and p38 Mitogen-Activated Protein Kinases Regulate Pluripotent Cell Differentiation in Culture
Synthetic Glycopolymers for Highly Efficient Differentiation of Embryonic Stem Cells into Neurons: Lipo- or Not?
Significant differences of function and expression of microRNAs between ground state and serum-cultured pluripotent stem cells
The endogenous regenerative capacity of the damaged newborn brain: boosting neurogenesis with mesenchymal stem cell treatment
Germ-line-competent embryonic stem cells of the Chinese Kunming mouse strain with long-term self-renewal ability
Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells
Long-Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation.
Integrative and perturbation-based analysis of the transcriptional dynamics of TGFβ/BMP system components in transition from embryonic stem cells to neural progenitors
A glimpse into molecular mechanisms of embryonic stem cells pluripotency: Current status and future perspective.
Phosphoproteomics identifies a bimodal EPHA2 receptor switch that promotes embryonic stem cell differentiation.
MicroRNA-dependent inhibition of PFN2 orchestrates ERK activation and pluripotent state transitions by regulating endocytosis.
Exploiting Heparan Sulfate Proteoglycans in Human Neurogenesis-Controlling Lineage Specification and Fate
Retinoic Acid Induces Differentiation of Mouse F9 Embryonic Carcinoma Cell by Modulating the miR-485 Targeting of Abhd2
Inhibition of MEK1/2 and GSK3 (2i system) affects blastocyst quality and early differentiation of porcine parthenotes
Spred negatively regulates lens growth by modulating epithelial cell proliferation and fiber differentiation
This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.
Adult Stem Cells
Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.