A dishevelled-1/Smad1 interaction couples WNT and bone morphogenetic protein signaling pathways in uncommitted bone marrow stromal cells.

The Journal of Biological Chemistry
Zhongyu LiuThomas L Clemens

Abstract

Genetic evidence from both humans and mice suggests that Wnt/beta-catenin and bone morphogenetic protein (BMP) signaling pathways are essential for bone marrow mesenchymal stem cells to differentiate into osteoblasts. Here we describe a mechanism through which BMPs antagonize Wnt signaling and retard bone marrow mesenchymal stem cell proliferation. Treatment with Wnt3a, but not BMP-2, stimulated Lef1-mediated transcriptional activity, whereas co-stimulation with both Wnt3a and BMP-2 markedly reduced Wnt3a-induced reporter activity. Immunoprecipitation assays in 293T cells transfected with individual Smads and Wnt pathway components revealed a specific interaction between Dvl-1 and Smad1 that was dependent on the presence of Wnt3a or BMP-2. Under unstimulated conditions, Dvl-1 and Smad1 are co-immunoprecipitated and form a complex through the linker region of Smad1. Wnt3a treatment transiently disrupted the Dvl-1/Smad1 interaction coincident with nuclear accumulation of beta-catenin. In contrast, when cells were exposed to both Wnt3a and BMP-2, there was an enhanced accumulation of the Dvl-1-Smad1 complex and a decreased nuclear accumulation of beta-catenin. Expression of a mutant Smad1 protein, which cannot be phosphorylated in...Continue Reading

References

Aug 26, 1998·Genes & Development·M Whitman
Oct 6, 1998·Annual Review of Biochemistry·J Massagué
Jan 8, 2000·The Journal of Biological Chemistry·X YangX Cao
Dec 6, 2000·European Journal of Biochemistry·S ItohP Ten Dijke
Jul 5, 2001·Molecular and Cellular Biology·M FuruhashiM Kato
Dec 18, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keiji MiyazawaKohei Miyazono
Jan 22, 2003·Biochemical and Biophysical Research Communications·Gerard BainJackie Papkoff
Feb 11, 2003·The Journal of Biological Chemistry·Liyu WuXu Cao
Mar 26, 2003·FEBS Letters·Dennis R WarnerRobert M Greene
May 2, 2003·The Journal of Bone and Joint Surgery. American Volume·Rocky S Tuan
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Georges RawadiSergio Roman-Roman
Jan 20, 2004·Trends in Cell Biology·Elaine S Seto, Hugo J Bellen
Jan 20, 2004·Journal of Cell Science·Manolis Fanto, Helen McNeill
Feb 10, 2004·Frontiers in Bioscience : a Journal and Virtual Library·Di ChenZenghui Mi
Mar 16, 2004·Science·Anna-Pavlina G HaramisHans Clevers
Mar 26, 2004·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shuanhu ZhouJulie Glowacki
Jul 24, 2004·Differentiation; Research in Biological Diversity·Assia DerfoulDavid J Hall
Oct 12, 2004·The Biochemical Journal·Rafal SwierczMark T Bedford
Oct 22, 2004·The Biochemical Journal·Thomas ArnesenJohan R Lillehaug
Nov 5, 2004·Journal of Cellular Biochemistry·Gabriel MbalavieleRoberto Civitelli
Nov 9, 2004·Current Opinion in Cell Biology·Maurice Kléber, Lukas Sommer
Feb 8, 2005·Biochemical and Biophysical Research Communications·Mei Wan, Xu Cao
Mar 12, 2005·Trends in Molecular Medicine·M Audrey Koay, Matthew A Brown
Mar 24, 2005·Journal of Cellular and Molecular Medicine·S J LeedhamN A Wright
Mar 31, 2005·The Journal of Biological Chemistry·Hui ZhengYves L Marcel
Apr 15, 2005·Nature·Tannishtha Reya, Hans Clevers
Apr 19, 2005·The Journal of Biological Chemistry·Ivo KalajzicDavid W Rowe
Apr 20, 2005·The Journal of Cell Biology·Maurice KléberLukas Sommer
Apr 28, 2005·Developmental Neuroscience·Terence J Van Raay, Monica L Vetter
May 14, 2005·Anatomy and Embryology·Corina Schmidt, Ketan Patel
Jun 14, 2005·Developmental Biology·Luis de la Fuente, Jill A Helms
Aug 16, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Koji Nakagawa, Noboru Kuzumaki
Aug 27, 2005·Oncogene·Alison C Varga, Jeffrey L Wrana
Aug 30, 2005·Gene·Xu Cao, Di Chen

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Citations

Jul 28, 2007·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Lawrence G Raisz
Apr 23, 2010·Cancer Metastasis Reviews·Scott A Bergfeld, Yves A DeClerck
Oct 20, 2006·Reviews in Endocrine & Metabolic Disorders·Jane B LianDaniel W Young
Nov 13, 2008·Cell Research·Xing Guo, Xiao-Fan Wang
Dec 31, 2008·Cell Research·Tetsuro Watabe, Kohei Miyazono
Jun 7, 2007·Current Opinion in Rheumatology·Mark L Johnson, Mohamed A Kamel
Jun 26, 2007·Cells, Tissues, Organs·Christoph A GebeshuberStefan Grunert
Jun 6, 2007·The Journal of Clinical Investigation·Ying WangThomas L Clemens
Feb 16, 2008·The Journal of Clinical Investigation·Wan-Heng WangAbbot F Clark
May 30, 2009·Blood·Hyung Chan SuhJonathan R Keller
Jan 14, 2009·The Journal of Cell Biology·Vinicio A de Jesus PerezMarlene Rabinovitch
Oct 11, 2011·Connective Tissue Research·Kevin A Tompkins
Mar 16, 2011·Experimental Cell Research·Razvan L MicleaMarcel Karperien
Nov 21, 2009·Cytokine & Growth Factor Reviews·Buer SongKaren M Lyons
Sep 11, 2009·Trends in Molecular Medicine·Frédéric DeschaseauxDominique Heymann
Dec 17, 2015·Rheumatology·Martijn H van den BoschPeter M van der Kraan
Jun 13, 2015·Scientific Reports·Stefan Karl, Thomas Dandekar
Jul 28, 2011·Journal of Cellular Biochemistry·Grace L Lin, Kurt D Hankenson
Jun 23, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Nobue Itasaki, Stefan Hoppler
Oct 1, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Haiming LeiJunjie Lu
Mar 31, 2015·Tissue Engineering. Part B, Reviews·Abhirath ParikhEmmanuel S Tzanakakis
Sep 11, 2012·Biochimica Et Biophysica Acta·Masayo Sakaki-YumotoRik Derynck
Feb 3, 2012·Archives of Oral Biology·Hao-yuan WanZhen-zhen Shang
Nov 5, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Khatereh Saei ArezoumandNosratollah Zarghami
Jan 12, 2017·Journal of Biomedical Materials Research. Part a·Ju-Suk NamSang-Soo Lee
Mar 22, 2013·Therapeutic Advances in Musculoskeletal Disease·Jeong Hwan KimTong-Chuan He
May 15, 2007·The Journal of Biological Chemistry·Sheila RydzielErnesto Canalis
Jan 23, 2019·Frontiers in Cell and Developmental Biology·Khosrow S HouschyarBjörn Behr
Nov 7, 2006·The Journal of Biological Chemistry·Yan ChenBenjamin A Alman
Feb 3, 2012·Physiological Reviews·Kirsty Greenow, Alan R Clarke

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