A disintegrin and metalloprotease 23 hypermethylation predicts decreased disease-free survival in low-risk breast cancer patients

Cancer Science
Iveta ZmetakovaIvana Fridrichova

Abstract

A Disintegrin And Metalloprotease 23 (ADAM23), a member of the ADAM family, is involved in neuronal differentiation and cancer. ADAM23 is considered a possible tumor suppressor gene and is frequently downregulated in various types of malignancies. Its epigenetic silencing through promoter hypermethylation was observed in breast cancer (BC). In the present study, we evaluated the prognostic significance of ADAM23 promoter methylation for hematogenous spread and disease-free survival (DFS). Pyrosequencing was used to quantify ADAM23 methylation in tumors of 203 BC patients. Presence of circulating tumor cells (CTC) in their peripheral blood was detected by quantitative RT-PCR. Expression of epithelial (KRT19) or mesenchymal (epithelial-mesenchymal transition [EMT]-inducing transcription factors TWIST1, SNAI1, SLUG and ZEB1) mRNA transcripts was examined in CD45-depleted peripheral blood mononuclear cells. ADAM23 methylation was significantly lower in tumors of patients with the mesenchymal CTC (P = .006). It positively correlated with Ki-67 proliferation, especially in mesenchymal CTC-negative patients (P = .001). In low-risk patients, characterized by low Ki-67 and mesenchymal CTC absence, ADAM23 hypermethylation was an independ...Continue Reading

References

Jan 1, 1995·Methods in Enzymology·N D Rawlings, A J Barrett
Aug 3, 2002·Nature Reviews. Cancer·Ann F ChambersIan C MacDonald
Oct 2, 2003·Current Opinion in Cell Biology·Judith M White
May 3, 2005·Neuroscience Letters·Fabrício F CostaAnamaria A Camargo
Feb 8, 2007·Cancer Genetics and Cytogenetics·Marilia F CalmonPaula Rahal
May 6, 2008·The Breast : Official Journal of the European Society of Mastology·R Stuart-HarrisP Pharoah
Sep 3, 2008·Molecular Aspects of Medicine·Dylan R EdwardsCaroline J Pennington
Dec 18, 2008·International Journal of Cancer. Journal International Du Cancer·Jin-Sung ChoiJae-Gahb Park
Jan 2, 2009·Journal of Cell Science·David S Harburger, David A Calderwood
Jun 3, 2009·The Journal of Clinical Investigation·Michael Zeisberg, Eric G Neilson
Jun 25, 2009·Cancer Research·Newton V VerbisckAnamaria A Camargo
Feb 8, 2011·Breast Cancer Research and Treatment·Cristina RaimondiPaola Gazzaniga
Mar 1, 2011·Journal of Cellular and Molecular Medicine·Angela GradilonePaola Gazzaniga
Mar 10, 2011·International Journal of Cancer. Journal International Du Cancer·Michal MegoJames M Reuben
Mar 25, 2011·International Journal of Experimental Pathology·Chunyan HuJianhua Zhou
Sep 13, 2011·Clinical Proteomics·Michael J DuffyPatricia M McGowan
May 9, 2013·British Journal of Cancer·A Q van HoeselD S B Hoon
Jul 13, 2013·Journal of Internal Medicine·A J Redig, S S McAllister
Jun 29, 2014·BMC Cancer·Zuzana CiernaJozef Mardiak
Aug 15, 2014·Developmental Cell·Jay S DesgrosellierDavid A Cheresh
Nov 16, 2014·EMBO Molecular Medicine·Simon A JoosseKlaus Pantel
Jan 27, 2015·Translational Research : the Journal of Laboratory and Clinical Medicine·Ivana FridrichovaLudovit Danihel
Jul 16, 2015·International Journal of Cancer. Journal International Du Cancer·Balázs GyőrffyLibero Santarpia
Aug 19, 2015·The Breast : Official Journal of the European Society of Mastology·Carsten DenkertFrederick Klauschen
Aug 21, 2015·Connective Tissue Research·Jacqueline Banyard, Diane R Bielenberg
Apr 7, 2016·Journal of the American College of Surgeons·Carolyn S HallAnthony Lucci
Jul 20, 2016·Journal of Clinical Medicine·Joema Felipe LimaJohn M S Bartlett
Sep 16, 2017·Molecular and Cellular Biochemistry·Lei WangLance C Bridges

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Citations

Aug 28, 2020·PloS One·Kajal DevaniTiago S Valente
Nov 7, 2019·Cells·Ivana Fridrichova, Iveta Zmetakova
May 6, 2021·Journal of Cellular and Molecular Medicine·Chao LiDidi Zuo

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Methods Mentioned

BETA
PCR

Software Mentioned

SPSS statistics
PyroMark Q24

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