A distant coilin homologue is required for the formation of cajal bodies in Arabidopsis.

Molecular Biology of the Cell
Sarah CollierPeter Shaw

Abstract

Cajal bodies (CBs) are subnuclear bodies that are widespread in eukaryotes, being found in mammals, many other vertebrates and in all plant species so far examined. They are mobile structures, moving, fusing, and budding within the nucleus. Here we describe a screen for Arabidopsis mutants with altered CBs and describe mutants that have smaller Cajal bodies (ncb-2, ncb-3), lack them altogether (ncb-1), have increased numbers of CBs (pcb) or have flattened CBs (ccb). We have identified the gene affected in the ncb mutants as a distant homolog of the vertebrate gene that encodes coilin (At1g13030) and have termed the resulting protein Atcoilin. A T-DNA insertional mutant in this gene (ncb-4) also lacks Cajal bodies. Overexpression of Atcoilin cDNA in ncb-1 restores Cajal bodies, which recruit U2B'' as in the wild type, but which are, however, much larger than in the wild type. Thus we have shown that At1g13030 is required for Cajal body formation in Arabidopsis, and we hypothesize that the level of its expression is correlated with Cajal body size. The Atcoilin gene is unaffected in pcb and ccb, suggesting that other genes can also affect CBs.

References

May 1, 1969·Journal of Ultrastructure Research·A Monneron, W Bernhard
Jan 1, 1995·Developmental Genetics·J G GallC Murphy
Aug 1, 1993·The Journal of Cell Biology·R S TumaM B Roth
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J G Gall
Dec 5, 2000·Molecular Biology of the Cell·M D Hebert, A G Matera
Jan 3, 2001·The Journal of Cell Biology·M PlataniA I Lamond
Jun 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert E CampbellRoger Y Tsien
Jun 19, 2002·Chromosoma·Ricardo AcevedoS Moreno Díaz de la Espina
Oct 4, 2002·Developmental Cell·Michael D HebertA Gregory Matera
Oct 16, 2002·The Journal of Cell Biology·Stephen C Ogg, Angus I Lamond
Dec 17, 2002·Journal of Cell Science·Karl B ShpargelMichael D Hebert
Apr 8, 2003·Journal of Cell Science·Judith E SleemanAngus I Lamond
Jul 5, 2003·Current Biology : CB·A Gregory Matera
Oct 7, 2003·Molecular Biology of the Cell·Yusheng ZhuMichael P Terns
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Joseph G Gall
Mar 17, 2004·The Journal of Cell Biology·Miroslav DundrTom Misteli
Jul 17, 2004·The EMBO Journal·Nina SchaffertReinhard Lührmann
Aug 19, 2004·Journal of Cell Science·Dobrila NesicAngela Krämer
Sep 29, 2004·The Journal of Cell Biology·David Stanĕk, Karla M Neugebauer
Oct 20, 2004·Current Opinion in Plant Biology·Peter J Shaw, John W S Brown
Dec 22, 2004·The Plant Journal : for Cell and Molecular Biology·Olga A KorolevaJohn H Doonan
Oct 11, 2005·Annual Review of Cell and Developmental Biology·Mario Cioce, Angus I Lamond

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Citations

Sep 13, 2011·Chromosoma·Dariusz Jan Smoliński, Agnieszka Kołowerzo
Sep 10, 2011·Histochemistry and Cell Biology·Dariusz J SmolińskiAlicja Górska-Brylass
Oct 16, 2008·Protoplasma·Janusz NiedojadłoElzbieta Bednarska
May 6, 2009·Protoplasma·Agnieszka KołowerzoElzbieta Bednarska
Nov 19, 2010·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Emma HeathGarry T Morgan
Nov 9, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Zehra F Nizami, Joseph G Gall
Mar 4, 2011·Cell Death & Disease·S MahmoudiM Farnebo
Apr 2, 2010·Nature Structural & Molecular Biology·Magdalena StrzeleckaKarla M Neugebauer
Apr 7, 2010·Nature Structural & Molecular Biology·Je-Hyun Yoon, Roy Parker
Jan 23, 2009·Molecular Biology of the Cell·Ji-Long LiuJoseph G Gall
Oct 23, 2009·Molecular Biology of the Cell·Svetlana Deryusheva, Joseph G Gall
Nov 14, 2012·Briefings in Functional Genomics·Nicolás G BolognaJavier F Palatnik
Aug 7, 2007·Plant & Cell Physiology·Yoichiro FujiokaYuichiro Watanabe
May 28, 2010·Cold Spring Harbor Perspectives in Biology·Zehra NizamiJoseph G Gall
Sep 29, 2011·Plant Physiology·Elise K Van BuskirkMeng Chen
Jan 30, 2010·International Journal of Cell Biology·K Zienkiewicz, E Bednarska
Jan 16, 2013·PloS One·Valentine MakarovNatalia Kalinina
Jun 19, 2013·PloS One·Olga PontesPedro Costa-Nunes
Jan 1, 2013·Case Reports in Hepatology·P R Thippeswamy NaikP Vinod Kumar
May 23, 2014·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Pedro Costa-NunesOlga Pontes
Mar 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·Liang SongNina Fedoroff
Jan 1, 2013·Journal of Molecular Biology·Hanna J Broome, Michael D Hebert
Jun 18, 2011·Trends in Genetics : TIG·Yuntao S MaoDavid L Spector
Apr 19, 2007·Biology of the Cell·Olga EcheverríaSusana Moreno Díaz de la Espina
Nov 5, 2008·The Plant Journal : for Cell and Molecular Biology·Robert BlanvillainDavid W Ow
Apr 6, 2007·The EMBO Journal·Sang Hyon KimMichael Taliansky
Oct 9, 2012·Wiley Interdisciplinary Reviews. RNA·Martin MachynaKarla M Neugebauer
Sep 7, 2012·Cell Biology International·Clara Pérez-MuniveSusana Moreno Díaz de la Espina
Mar 3, 2010·Archives of Biochemistry and Biophysics·Michael D Hebert
Mar 24, 2016·RNA Biology·Janusz NiedojadłoKatarzyna Niedojadło
May 15, 2015·RNA Biology·Martin MachynaDavid Staněk
Apr 25, 2006·Current Opinion in Cell Biology·A Gregory Matera, Karl B Shpargel
Feb 18, 2011·Nucleus·Magdalena StrzeleckaKarla M Neugebauer
Jan 21, 2015·Cell Reports·Ivan NovotnýDavid Staněk
Nov 20, 2009·Developmental Cell·A Gregory MateraT K Rajendra
Jun 4, 2013·Biochemical and Biophysical Research Communications·Martin SvédaZdeněk Knejzlík
Aug 28, 2014·Frontiers in Plant Science·Tongtong Guo, Yuda Fang
Nov 5, 2014·PloS One·Janusz NiedojadłoDariusz J Smoliński

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