A distinct gene expression signature characterizes human neuroblastoma cancer stem cells

Stem Cell Research
Robert A RossNai-Kong V Cheung

Abstract

Neuroblastoma, a malignancy of multipotent embryonic neural crest cells, is the most common extracranial solid cancer in childhood and most common cancer in infancy. Cellular phenotype has been shown to be an important determinant of the malignant potential in human neuroblastoma cells and tumors. Whereas neuroblastic (N-type) are moderately malignant and nonneuronal (S-type) cells are nonmalignant, I-type stem cells are highly tumorigenic, irrespective of N-myc amplification status. In the present study, we sought to determine which genes were overexpressed in the I-type cells which might characterize and maintain the stem cell state and/or malignancy of human neuroblastoma cancer stem cells. We used a microarray platform to compare the steady-state expression levels of mRNAs from 13 human neuroblastoma cell lines representing the three cellular phenotypes. Using qRT-PCR and Western blot analyses, we identified seven genes whose expression is consistently elevated exclusively in neuroblastoma cancer stem cells: CD133, KIT, NOTCH1, GPRC5C, PIGF2, TRKB, and LNGFR. Moreover, we show that the genes are phenotype specific, as differentiation of I-type BE(2)-C cells to either an N- or S-type morphology results in significantly reduc...Continue Reading

References

Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·N IkegakiR H Kennett
Dec 20, 2000·Proceedings of the National Academy of Sciences of the United States of America·N UchidaI L Weissman
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Dec 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Houman D HemmatiHarley I Kornblum
Jan 22, 2004·Archives of Medical Research·Albertus W WognumTerry E Thomas
Aug 4, 2004·Seminars in Cancer Biology·Sven PåhlmanHåkan Axelson
Feb 22, 2005·Neoplasia : an International Journal for Oncology Research·Jeanette D WaltonRobert A Ross
Aug 11, 2005·PLoS Biology·Craig E EckfeldtCatherine M Verfaillie
May 25, 2006·Biology of the Cell·Jianping ZhangYan Jin
Jul 15, 2006·Seminars in Cancer Biology·Robert A Ross, Barbara A Spengler
Aug 12, 2006·Brain : a Journal of Neurology·H LiuJ D Kocsis
Apr 12, 2008·World Journal of Pediatrics : WJP·Qiang-Song TongJia-Bin Cai
Mar 6, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Itaru ImayoshiRyoichiro Kageyama
Jan 10, 2012·Experimental & Molecular Medicine·Sandro De Falco
Mar 7, 2013·Endocrine-related Cancer·Georg HilfenhausChristian Fischer
May 25, 2013·Nature Reviews. Cancer·Nai-Kong V Cheung, Michael A Dyer

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Citations

Dec 24, 2015·Frontiers in Cell and Developmental Biology·Helen ForghamJane Carr-Wilkinson
Feb 26, 2016·Cancer Biotherapy & Radiopharmaceuticals·Liqun YangYundong Zhang
Apr 5, 2016·Annals of Neurology·Nancy RatnerNina F Schor
Sep 27, 2016·International Journal of Molecular Sciences·Karin ZinsDietmar Abraham
Dec 10, 2017·Cell and Tissue Research·Julie A TomolonisJason M Shohet
Jun 5, 2019·Neurotoxicity Research·Konrad A SzychowskiJan Gmiński
Aug 18, 2018·Journal of Immunology Research·Irina V KholodenkoRoman V Kholodenko
Jun 14, 2019·Frontiers in Molecular Neuroscience·Hisham F BahmadWassim Abou-Kheir
Feb 21, 2019·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Thatyanne Gradowski do NascimentoSelene Elifio-Esposito
Dec 6, 2019·Frontiers in Endocrinology·Veronica VeschiCarol J Thiele
Dec 18, 2020·Frontiers in Cell and Developmental Biology·John C NolanOlga Piskareva
Dec 8, 2016·Cancer Research·MoonSun JungMichelle J Henderson
Jan 29, 2019·Journal of the American Chemical Society·Fengfei WangErxi Wu
Aug 14, 2020·Experimental Biology and Medicine·Sheron Campos CogoSelene Elifio-Esposito
Aug 11, 2021·Cellular and Molecular Neurobiology·Konrad A SzychowskiAnna K Wójtowicz

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