A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus

The American Journal of Physiology
T D SingerC F Higgins

Abstract

The killifish, Fundulus heteroclitus, is a euryhaline teleost fish capable of adapting rapidly to transfer from freshwater (FW) to four times seawater (SW). To investigate osmoregulation at a molecular level, a 5.7-kilobase cDNA homologous to human cystic fibrosis transmembrane conductance regulator (hCFTR) was isolated from a gill cDNA library from SW-adapted killifish. This cDNA encodes a protein product (kfCFTR) that is 59% identical to hCFTR, the most divergent form of CFTR characterized to date. Expression of kfCFTR in Xenopus oocytes generated adenosine 3',5'-cyclic monophosphate-activated, Cl(-)-selective currents similar to those generated by hCFTR. In SW-adapted killifish, kfCFTR was expressed at high levels in the gill, opercular epithelium, and intestine. After abrupt exposure of FW-adapted killifish to SW, kfCFTR expression in the gill increased severalfold, suggesting a role for kfCFTR in salinity adaptation. Under similar conditions, plasma Na+ levels rose significantly after 8 h and then fell, although it is not known whether these changes are directly responsible for the changes in kfCFTR expression. The killifish provides a unique opportunity to understand teleost osmoregulation and the role of CFTR.

References

Jul 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·P M Quinton
Aug 1, 1989·The American Journal of Physiology·M A GrayB E Argent
Feb 1, 1989·The Journal of Cell Biology·M Kozak
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·S J TebbuttA Harris
Mar 1, 1993·Human Molecular Genetics·A E TreziseA Harris
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W S MarshallD Garg
Nov 1, 1996·The Journal of General Physiology·J B Sørensen, E H Larsen
Jan 1, 1997·The Journal of Physiology·F M GribbleF M Ashcroft

❮ Previous
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Citations

May 16, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Feng PanTerence M Bradley
Mar 27, 2004·American Journal of Physiology. Cell Physiology·Graham R ScottPatricia M Schulte
Jun 6, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David H Evans
Nov 15, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·John B Pritchard
Jul 13, 2002·The Journal of Experimental Zoology·James B ClaiborneAlison I Morrison-Shetlar
Oct 29, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·R J Gonzalez
Oct 3, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Kelly A Hyndman, David H Evans
Jun 14, 2016·Conservation Physiology·D Ferreira-MartinsJ M Wilson
Sep 5, 2017·Nature Reviews. Nephrology·Marcel H A MuskietDaniël H van Raalte
Sep 6, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Andre P SealeDarren T Lerner
Jan 25, 2000·American Journal of Physiology. Cell Physiology·M Baudouin-LegrosA Edelman
Dec 31, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Fumi KatohGreg G Goss
Dec 18, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Brian S ShepherdMathilakath M Vijayan
Dec 17, 2009·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·K J DemmersA G Butt
Apr 2, 2008·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Chris M Wood, Martin Grosell
Aug 8, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ilan M RuhrMartin Grosell
Jul 19, 2018·Frontiers in Physiology·Salman Malakpour KolbadinezhadJonathan M Wilson
Dec 20, 2019·Clinical Journal of Sport Medicine : Official Journal of the Canadian Academy of Sport Medicine·Eileen BridgesTamara Hew-Butler
Sep 24, 2011·American Journal of Physiology. Cell Physiology·Maximilian StahlJohn N Forrest
Nov 1, 2015·Biochemical Society Transactions·Samuel J BoseDavid N Sheppard
Dec 20, 2017·Zoological Letters·Marty Kwok Shing WongYoshio Takei
Oct 28, 2015·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Richard T Di Giulio, Bryan W Clark
Apr 1, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ilan M RuhrMartin Grosell
May 29, 2015·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ilan M RuhrMartin Grosell
Aug 13, 2005·The Journal of Biological Chemistry·Daniel LingwoodJames S Ballantyne
Jan 26, 2018·Clinical Journal of Sport Medicine : Official Journal of the Canadian Academy of Sport Medicine·Eileen BridgesTamara Hew-Butler
Jan 17, 2021·Journal of Fish Biology·Luis Vargas-ChacoffJorge M Navarro
Feb 5, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Adalto BianchiniEuclydes Antônio Dos Santos Filho
Nov 14, 2020·Journal of Experimental Zoology. Part A, Ecological and Integrative Physiology·Ciaran A Shaughnessy, Jason P Breves
Oct 14, 2018·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K Keano PavloskyAndre P Seale
Jul 16, 2008·The Journal of Experimental Biology·Graham R ScottChris M Wood
Nov 12, 2003·The Journal of Experimental Biology·Jeff G RichardsPatricia M Schulte

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