A DNA barcode library for ground beetles of Germany: the genus Amara Bonelli, 1810 (Insecta, Coleoptera, Carabidae)

ZooKeys
Michael J RaupachLars Hendrich

Abstract

The genus Amara Bonelli, 1810 is a very speciose and taxonomically difficult genus of the Carabidae. The identification of many of the species is accomplished with considerable difficulty, in particular for females and immature stages. In this study the effectiveness of DNA barcoding, the most popular method for molecular species identification, was examined to discriminate various species of this genus from Central Europe. DNA barcodes from 690 individuals and 47 species were analysed, including sequences from previous studies and more than 350 newly generated DNA barcodes. Our analysis revealed unique BINs for 38 species (81%). Interspecific K2P distances below 2.2% were found for three species pairs and one species trio, including haplotype sharing between Amara alpina/Amara torrida and Amara communis/Amara convexior/Amara makolskii. This study represents another step in generating an extensive reference library of DNA barcodes for carabids, highly valuable bioindicators for characterizing disturbances in various habitats.

References

Jul 13, 2000·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Z ZhangW Miller
Oct 26, 2000·Molecular Ecology·M ClementK A Crandall
May 23, 2001·Trends in Ecology & Evolution·D BensassonG M. Hewitt
Mar 5, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Sep 4, 2003·Proceedings. Biological Sciences·Paul D N HebertJeremy R deWaard
Jan 1, 1996·Annual Review of Entomology·G L Lövei, K D Sunderland
Aug 17, 2005·Molecular Ecology·M H GreenstoneS A Rehner
Jan 19, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mehrdad HajibabaeiPaul D N Hebert
Mar 9, 2007·Frontiers in Zoology·Martin Wiemers, Konrad Fiedler
Jun 24, 2008·Bioinformatics·Aleksandr MorgulisAlejandro A Schäffer
Jun 25, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Lise Frézal, Raphael Leblois
Jul 23, 2008·Methods in Molecular Biology·Jeremy R deWaardPaul D N Hebert
Sep 12, 2008·Molecular Ecology Notes·Sujeevan Ratnasingham, Paul D N Hebert
Sep 17, 2008·Nature Reviews. Microbiology·John H WerrenMichael E Clark
Dec 17, 2009·Frontiers in Zoology·M Alex Smith, Brian L Fisher
Mar 25, 2011·Molecular Ecology Resources·Sunghoon JungSeunghwan Lee
Apr 5, 2011·Molecular Ecology Resources·Matthew H GreenstoneJing H Hu
Jan 1, 2010·Molecular Ecology Resources·R H GloverN Boonham
Mar 9, 2012·Systematic Biology·Johannes BergstenAlfried P Vogler
Apr 11, 2012·Molecular Ecology·Pierre TaberletEske Willerslev
Jul 13, 2012·Trends in Ecology & Evolution·Brett R ScheffersWilliam F Laurance
Jan 3, 2013·Molecular Ecology Resources·R A Collins, R H Cruickshank
Jul 19, 2013·PloS One·Sujeevan Ratnasingham, Paul D N Hebert
Oct 30, 2013·BMC Ecology·Thomas S WoodcockSarah J Adamowicz
Jan 24, 2014·ZooKeys·Mansour AliabadianRonald Vonk
Mar 13, 2014·Molecular Ecology Resources·Thomas KnebelsbergerFilipe O Costa
Jul 22, 2014·Molecular Ecology Resources·Klemen Čandek, Matjaž Kuntner
Oct 7, 2014·PloS One·Jérome MorinièreAxel Gruppe
Dec 4, 2014·Trends in Ecology & Evolution·W John KressDavid L Erickson
Jan 31, 2015·PloS One·Yuichi ObaYoshinori Kumazawa

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Citations

Jun 20, 2020·Molecular Ecology Resources·Laura A HardulakGerhard Haszprunar
Jun 4, 2020·Ecology and Evolution·Axel HausmannJan Christian Habel
Dec 11, 2019·PloS One·Muhammad Tayyib NaseemPaul D N Hebert

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Datasets Mentioned

BETA
MH300683
AAJ5377

Methods Mentioned

BETA
PCR

Software Mentioned

PopART
BOLD aligner
Geneious
MEGA7
MUSCLE
BLAST
nBLAST
BOLD workbench
BOLD

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